05-1243 | Anti-Sirt1 Antibody, clone 3H10.2

05-1243
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1243
      Replaces05-707
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Sirt1 Antibody, clone 3H10.2
      Background InformationThe Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53. There are now five described human homologs of yeast Sir2, which are named SIRT1-5.
      SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress. SIRT1 is a HDAC which is important in establishing repressive chromatin structures and plays a role in increasing lifespan.
      References
      Product Information
      FormatPurified
      PresentationPurified in PBS with 0.05% Sodium Azide
      Applications
      ApplicationAnti-Sirt1 Antibody, clone 3H10.2 is a Mouse Monoclonal Antibody for detection of Sirt1 also known as Sirtuin 1, Silent mating type Information Regulation-2 & has been tested in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to amino acids 508-616 of human Sirt1.
      Clone3H10.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityHuman Sirt1. Does not cross react with lysates from mouse NIH/3T3 cells or rat PC12 cells.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family.
      Gene Symbol
      • SIRT1
      • SIR2L1
      • hSIR2
      • OTTHUMP00000060745
      • hSIRT1
      • SIR2alpha
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q96EB6 # NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2- mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein.
      COFACTOR: Binds 1 zinc ion per subunit (By similarity).
      SIZE: 747 amino acids; 81681 Da
      SUBUNIT: Interacts with TAF1B. Found in a complex with PCAF and MYOD1 (By similarity). Interacts with MLLT7/FOXO4, HES1, HEY2, p53/TP53 and PML.
      SUBCELLULAR LOCATION: Nucleus. Note=Recruited to the nuclear bodies via its interaction with PML.TISSUE SPECIFICITY: Widely expressed.
      SIMILARITY: SwissProt: Q96EB6 ## Belongs to the sirtuin family. & Contains 1 deacetylase sirtuin-type domain.
      MISCELLANEOUS: Red wine, which contains resveratrol, may participate in activation of sirtuin proteins, and may therefore participate in an extended lifespan as it has been observed in yeast.
      Molecular Weight120 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on nuclear extract from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8 °C in undiluted aliquots for up to 6 months after date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Sirt1, clone 3H10.2 - 2325107 2325107
      Anti-Sirt1, clone 3H10.2 - 2032739 2032739
      Anti-Sirt1, clone 3H10.2 - 2115993 2115993
      Anti-Sirt1, clone 3H10.2 - 2203070 2203070
      Anti-Sirt1, clone 3H10.2 - 2512504 2512504
      Anti-Sirt1, clone 3H10.2 - DAM1496614 DAM1496614
      Anti-Sirt1, clone 3H10.2 - DAM1538408 DAM1538408
      Anti-Sirt1, clone 3H10.2 - JBC1771109 JBC1771109
      Anti-Sirt1, clone 3H10.2 - NG1696469 NG1696469
      Anti-Sirt1, clone 3H10.2 - NG1734226 NG1734226

      References

      Reference overviewPub Med ID
      Sirtuins: novel targets for metabolic disease.
      Elliott, Peter J and Jirousek, Michael
      Current opinion in investigational drugs (London, England : 2000), 9: 371-8 (2008)  2008

      Show Abstract
      18393104 18393104
      p53 Activation: a case against Sir.
      Brooks, Christopher L and Gu, Wei
      Cancer Cell, 13: 377-8 (2008)  2008

      18455119 18455119
      Sirt1 contributes critically to the redox-dependent fate of neural progenitors.
      Prozorovski, Timour, et al.
      Nat. Cell Biol., 10: 385-94 (2008)  2008

      Show Abstract
      18344989 18344989
      hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase.
      Vaziri, H, et al.
      Cell, 107: 149-59 (2001)  2001

      Show Abstract
      11672523 11672523

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies