04-889 | Anti-Src Antibody, clone N6L, rabbit monoclonal

04-889
100 µl  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-889
      Replaces05-889
      DescriptionAnti-Src Antibody, clone N6L, rabbit monoclonal
      Alternate Names
      • proto-oncogene tyrosine-protein kinase SRC
      • protooncogene SRC, Rous sarcoma
      • tyrosine kinase pp60c-src
      • tyrosine-protein kinase SRC-1
      • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene
        homolog
      • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
        (avian)
      Background Informationpp60c-Src is the prototype non-receptor tyrosine kinase. Homology to Src in three regions is shared by a number of proteins: SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain. The Src SH2 domain binds to phospho-Tyr527 (phosphorylated by Csk) within the Src molecule, causing Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Included positive antigen control of non-stimulated A431 lysate (Cat. No. 12-301). Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationCultured supernantant containing 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Src Antibody, clone N6L is validated for use in WB for the detection of Src.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenN-terminal 6His-tagged recombinant full-length human Src expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.
      CloneN6L
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Src. Crossreacts with Yes and weakly with Fyn. No crossreactivity observed with Lck or Lyn.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.
      Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • ASV
      • EC 2.7.10.2
      • OTTHUMP00000174476
      • OTTHUMP00000174477
      • SRC1
      • c-SRC
      • c-Src
      • c-src
      • p60-Src
      • pp60c-src
      UniProt Number
      UniProt SummaryCATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
      SUBUNIT STRUCTURE: Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 By similarity. Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain. Interacts with CAV2 (tyrosine phosphorylated form).
      PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.

      Molecular Weight~ 60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: A 1:500 to 1:1000 dilution of this antibody detected Src in RIPA lysates from A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies