07-577 | Anti-Stargazin/Cacng2 Antibody, CT

07-577
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-577
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Stargazin/Cacng2 Antibody, CT
      Alternate Names
      • Neuronal voltage-gated calcium channel gamma-2 subunit
      • calcium channel, voltage-dependent, gamma subunit 2
      • voltage-dependent calcium channel gamma-2 subunit
      Background InformationStargazin belongs to a group called voltage gated calcium channels (VGCCs) which are present in most excitable cells. Stargazin is thought to stabilize the calcium channel in an inactivated (closed) state.
      There are five high voltage activated calcium channel types (L, N, P, Q, and R) and one low voltage activated channel type (T). Each of these channels exists as a heteromultimer of a1, b, a2/ d and g subunits with the voltage activated calcium channel function carried by the a subunits. VGCCs exert spatial and temporal control over cellular calcium concentrations and serve to modulate neurotransmitter release, hormone secretion, muscle contraction, electrical activity, cell metabolism and proliferation, gene expression, and neuronal survival.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
      Applications
      ApplicationThis Anti-Stargazin/Cacng2 Antibody, CT is validated for use in IC, IP, WB for the detection of Stargazin/Cacng2.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      2-5 µg of this antibody has been reported by an independent laboratory to immunoprecipitate Stargazin in rat P2 lysate.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 305-323 (KDSKDSLHANTANRRTTPV) of rat Stargazin.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Stargazin, MW ~36-39 kDa.
      IsotypeIgG
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteRat. Predicted to cross-react with mouse based on sequence similarity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryL-type calcium channels are composed of five subunits. The protein encoded by this gene represents one of these subunits, gamma, and is one of several gamma subunit proteins. It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state. This protein is similar to the mouse stargazin protein, mutations in which have been associated with absence seizures, also known as petit-mal or spike-wave seizures. This gene is a member of the neuronal calcium channel gamma subunit gene subfamily of the PMP-22/EMP/MP20 family.
      Gene Symbol
      • CACNG2
      • MGC138502
      • MGC138504
      • stargazin
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9Y698 # Thought to stabilize the calcium channel in an inactivated (closed) state.
      SIZE: 323 amino acids; 35966 Da
      SUBUNIT: The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Interacts with the PDZ domains of DLG4/PSD-95 and DLG1/SAP97. May interact with GOPC (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Brain.
      PTM: Phosphorylation of Thr-321 impairs interaction with DLG1 and DLG4.
      SIMILARITY: SwissProt: Q9Y698 ## Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.
      Molecular Weight~36-39 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).

      Western Blot Analysis:
      0.5-2.0 µg/mL of this antibody detected Stargazin in RIPA lysates from rat brain microsomal preparation (Catalog # 12-144).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Stargazin/Cacng2, CT - 2424771 2424771
      Anti-Stargazin/Cacng2, CT - 2039893 2039893
      Anti-Stargazin/Cacng2, CT - 2040084 2040084
      Anti-Stargazin/Cacng2, CT - 26934 26934
      Anti-Stargazin/Cacng2, CT - DAM1754443 DAM1754443
      Anti-Stargazin/Cacng2, CT - DAM1821148 DAM1821148
      Anti-Stargazin/Cacng2, CT - JBC1887184 JBC1887184
      Anti-Stargazin/Cacng2, CT -2512451 2512451
      Anti-Stargazin/Cacng2, CT -2680550 2680550
      Anti-Stargazin/Cacng2, CT -2724357 2724357

      References

      Reference overviewApplicationPub Med ID
      In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10 (zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting to Rab5-positive endosomes.
      Oku, S; Takahashi, N; Fukata, Y; Fukata, M
      The Journal of biological chemistry  288  19816-29  2013

      Show Abstract
      23687301 23687301
      Calpain-mediated regulation of stargazin in adult rat brain.
      Yu, L; Rostamiani, K; Hsu, YT; Wang, Y; Bi, X; Baudry, M
      Neuroscience  178  13-20  2011

      Show Abstract Full Text Article
      21256931 21256931
      Cornichon-2 modulates AMPA receptor-transmembrane AMPA receptor regulatory protein assembly to dictate gating and pharmacology.
      Gill, MB; Kato, AS; Roberts, MF; Yu, H; Wang, H; Tomita, S; Bredt, DS
      The Journal of neuroscience : the official journal of the Society for Neuroscience  31  6928-38  2011

      Show Abstract
      21543622 21543622
      PSD-95 and PSD-93 play critical but distinct roles in synaptic scaling up and down.
      Sun, Q; Turrigiano, GG
      The Journal of neuroscience : the official journal of the Society for Neuroscience  31  6800-8  2011

      Show Abstract
      21543610 21543610
      Tumor necrosis factor-α signaling maintains the ability of cortical synapses to express synaptic scaling.
      Steinmetz, CC; Turrigiano, GG
      The Journal of neuroscience : the official journal of the Society for Neuroscience  30  14685-90  2010

      Show Abstract
      21048125 21048125
      New transmembrane AMPA receptor regulatory protein isoform, gamma-7, differentially regulates AMPA receptors.
      Kato, AS; Zhou, W; Milstein, AD; Knierman, MD; Siuda, ER; Dotzlaf, JE; Yu, H; Hale, JE; Nisenbaum, ES; Nicoll, RA; Bredt, DS
      The Journal of neuroscience : the official journal of the Society for Neuroscience  27  4969-77  2007

      Show Abstract
      17475805 17475805
      Stargazin and other transmembrane AMPA receptor regulating proteins interact with synaptic scaffolding protein MAGI-2 in brain.
      Deng, F; Price, MG; Davis, CF; Mori, M; Burgess, DL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  26  7875-84  2006

      Show Abstract
      16870733 16870733
      The alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate receptor trafficking regulator "stargazin" is related to the claudin family of proteins by Its ability to mediate cell-cell adhesion.
      Price, Maureen G, et al.
      J. Biol. Chem., 280: 19711-20 (2005)  2005

      Show Abstract
      Immunocytochemistry15760900 15760900
      Nonchannel functions of the calcium channel gamma subunit: insight from research on the stargazer mutant.
      Qiao, Xiaoxi and Meng, Hongdi
      J. Bioenerg. Biomembr., 35: 661-70 (2003)  2003

      Show Abstract
      15000526 15000526
      Stargazin regulates synaptic targeting of AMPA receptors by two distinct mechanisms.
      Chen, L, et al.
      Nature, 408: 936-43 (2000)  2000

      Show Abstract
      11140673 11140673

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies