05-1100 | Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2

05-1100
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, IP, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1100
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2
      Background InformationSulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferase enzymes in the trans-Golgi-network. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosines in the total protein content of a cell can be sulfated. Sulfotyrosines mediates many receptor-ligand binding interactions, including the interaction of P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses; for example, tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells, and tyrosine sulfation of the Duffy antigen/receptor for chemokines is crucial for erythrocyte invasion by the malaria parasite Plasmodium vivax.
      This clone was derived from the antibody described in Kehoe et al., 2006.
      References
      Product Information
      FormatPurified
      Presentation100 μl in 100 mM Tris, 150 mM NaCl, 180 mM Glycine, pH 7.5 containing 0.05% sodium azide.
      Applications
      ApplicationDetect Sulfotyrosine with Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2 (Mouse Monoclonal Antibody), that has been shown to work in WB, IP, ELISA.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • ELISA
      Application NotesWB, IP, ELISA
      Biological Information
      ImmunogenThis antibody was developed using a phage display library as described in Kehoe et al., 2006. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC
      EpitopeSulfated Tyrosine
      CloneSulfo-1C-A2
      HostMouse
      SpecificityRecognizes sulfated tyrosine residues on various proteins. Does not recognize phosphotyrosine or unmodified tyrosines.
      IsotypeIgG2aκ
      Species Reactivity
      • All
      Species Reactivity NoteBroad species reactivity. Tested using human, mouse, bovine,and porcine, but the antibody is predicted to recognize any sulfotyrosine, regardless of surrounding amino acid sequence.
      Antibody TypeMonoclonal Antibody
      Purification MethodCulture supernatant, purified by protein G affinity chromatography
      Molecular Weightn/a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceAssayed using sulfatase treated bovine fibrinogen vs. untreated fibrinogen.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year from date of receipt when stored at +2-8°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - 2092739 2092739
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - 2020301 2020301
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - 2041601 2041601
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - 2187395 2187395
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - 2224443 2224443
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - 2361824 2361824
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - DAM1479644 DAM1479644
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - DAM1483555 DAM1483555
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - JBC1771084 JBC1771084
      Anti-Sulfotyrosine, clone Sulfo-1C-A2 - JBC1793799 JBC1793799

      References

      Reference overviewApplicationPub Med ID
      Tyrosylprotein sulfotransferase-1 and tyrosine sulfation of chemokine receptor 4 are induced by Epstein-Barr virus encoded latent membrane protein 1 and associated with the metastatic potential of human nasopharyngeal carcinoma.
      Xu, J; Deng, X; Tang, M; Li, L; Xiao, L; Yang, L; Zhong, J; Bode, AM; Dong, Z; Tao, Y; Cao, Y
      PloS one  8  e56114  2013

      Show Abstract
      Western Blotting23472069 23472069
      Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.
      Friedman, James, et al.
      PLoS ONE, 7: e37648 (2012)  2012

      Show Abstract
      22624058 22624058
      Structure and function of broadly reactive antibody PG16 reveal an H3 subdomain that mediates potent neutralization of HIV-1.
      Pejchal, Robert, et al.
      Proc. Natl. Acad. Sci. U.S.A., 107: 11483-8 (2010)  2010

      Show Abstract
      20534513 20534513
      Tyrosine sulfation of the amino terminus of PSGL-1 is critical for enterovirus 71 infection.
      Nishimura, Yorihiro, et al.
      PLoS Pathog., 6: e1001174 (2010)  2010

      Show Abstract
      21079683 21079683

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.