05-168 | Anti-TNFα Antibody, clone MPG-XT3

05-168
1 mg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MNEUTRPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-168
      Replaces04-1114
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-TNFα Antibody, clone MPG-XT3
      OverviewThe concentration of antibody needed to neutralize the bioactivity of TNF-α will depend on the cell type, the incubation conditions, and the biological response studied.
      References
      Product Information
      FormatPurified
      Presentation0.01M Tris-glycine, 0.15M NaCl, pH 7.4
      Applications
      ApplicationThis Anti-TNFalpha Antibody, clone MPG-XT3 is validated for use in NEUT for the detection of TNFα.
      Key Applications
      • Neutralizing
      Biological Information
      ImmunogenRecombinant murine TNF-α
      Cloneclone MPG-XT3
      HostRat
      Specificitymouse TNF-α; does not recognize human or rat TNF-α
      IsotypeIgG1
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
      Gene Symbol
      • TNF
      • TNFSF2
      • Cachectin
      • cachectin
      • TNF-alpha
      • DIF
      • OTTHUMP00000037669
      • TNFA
      • TNF-a
      Purification MethodDEAE chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
      SIZE: 233 amino acids; 25644 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
      PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
      SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely used to neutralize 50% of the cytotoxic effect of recombinant mouse TNF-α on actinomycin D treated L929 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-TNFa, clone MPG-XT3 (rat monoclonal IgG1) - 2045874 2045874
      Anti-TNFa, clone MPG-XT3 - 2060595 2060595
      Anti-TNFalpha;, clone MPG-XT3 - 14225 14225
      Anti-TNFalpha;, clone MPG-XT3 - 16940 16940
      Anti-TNFalpha;, clone MPG-XT3 - 22654 22654
      Anti-TNFalpha;, clone MPG-XT3 - 32350 32350
      Anti-TNFα, clone MPG-XT3 (rat monoclonal IgG1) - 2430418 2430418
      Anti-TNFα, clone MPG-XT3 -2543682 2543682
      Anti-TNFα, clone MPG-XT3 -2638016 2638016

      References

      Reference overviewApplicationPub Med ID
      Cellular stress amplifies TLR3/4-induced CXCL1/2 gene transcription in mononuclear phagocytes via RIPK1.
      Zhao, C; Pavicic, PG; Datta, S; Sun, D; Novotny, M; Hamilton, TA
      Journal of immunology (Baltimore, Md. : 1950)  193  879-88  2014

      Show Abstract
      24920846 24920846
      Activation of PPARbeta/delta causes a psoriasis-like skin disease in vivo.
      Romanowska, M; Reilly, L; Palmer, CN; Gustafsson, MC; Foerster, J
      PloS one  5  e9701  2010

      Show Abstract
      20300524 20300524
      A Kunitz-type protease inhibitor, bikunin, inhibits ovarian cancer cell invasion by blocking the calcium-dependent transforming growth factor-beta 1 signaling cascade
      Kobayashi, H., et al
      J Biol Chem, 278:7790-9 (2003)  2003

      Neutralization Assay12496270 12496270
      Role of laminin-1, collagen IV, and an autocrine factor(s) in regulated secretion by lacrimal acinar cells.
      L Chen, J D Glass, S C Walton, G W Laurie
      The American journal of physiology  275  C278-84  1998

      Show Abstract
      9688859 9688859
      Dermatophagoides-farinae-induced pulmonary eosinophilic inflammation in mice.
      Yu, C K, et al.
      Int. Arch. Allergy Immunol., 112: 73-82 (1997)  1997

      Show Abstract
      8980467 8980467
      Cytokine mRNA in the central nervous system of SCID mice infected with Toxoplasma gondii: importance of T-cell-independent regulation of resistance to T. gondii.
      Hunter, C A, et al.
      Infect. Immun., 61: 4038-44 (1993)  1993

      8406791 8406791

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      Life Science Research > Antibodies and Assays > Primary Antibodies