06-574 | Anti-TrkA Antibody

06-574
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-574
      ReplacesCBL770
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-TrkA Antibody
      Alternate Names
      • Neurotrophic tyrosine kinase receptor type
      • Oncogene TRK
      • TRK1-transforming tyrosine kinase protein
      • high affinity nerve growth factor receptor
      • neurotrophic tyrosine kinase, receptor, type 1
      • tyrosine kinase receptor A
      Background InformationTrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.
      References
      Product Information
      FormatPurified
      Control
      • EGF-stimulated A431 cel lysate, rat brain tissue. Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified rabbit IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at -20°C.
      Applications
      ApplicationAnti-TrkA Antibody detects level of TrkA & has been published & validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of a previous lot immunoprecipitated TrkA from 500 μg of PC-12 RIPA lysate.

      Immunocytochemistry:
      10 μg/mL of a previous lot of this antibody showed positive immunostaining for TrkA in PC-12 cells fixed with 4% paraformaldehyde.
      Biological Information
      ImmunogenRabbits immunized with bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes TrkA, Mr 140 kDa. Does not cross-react with TrkB or TrkC.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteRat, mouse and human. Reactivity with other species has not been confirmed.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.
      Gene Symbol
      • DKFZp781I14186
      • EC 2.7.10.1
      • MTC
      • OTTHUMP00000038736
      • TRK
      • TRK1
      • TRKA
      • Trk-A
      • p140-TrkA
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04629 # Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.
      SIZE: 796 amino acids; 87497 Da
      SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). Note=Endocytosed to the endosomes upon treatment of cells with NGF (By similarity).
      TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells; isoform TrkA-I is found in non-neuronal tissues.
      PTM: Ligand-mediated auto-phosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent.
      DISEASE: SwissProt: P04629 # Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis (CIPA) [MIM:256800]. CIPA is characterized by a congenital insensitivity to pain, anhidrosis (absence of sweating), absence of reaction to noxious stimuli, self-mutilating behavior, and mental retardation. This rare autosomal recessive disorder is also known as congenital sensory neuropathy with anhidrosis or hereditary sensory and autonomic neuropathy type IV or familial dysautonomia type II. & Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1; a rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1. & Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein.
      SIMILARITY: SwissProt: P04629 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 3 LRR (leucine-rich) repeats. & Contains 1 protein kinase domain. ... hide » see more »
      Molecular Weight140 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on RIPA lysates from human A431 cells, mouse 3T3 or rat PC-12 cell lysates.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected TrkA in RIPA lysates from human A431 cells; a previous lot detected TrkA in mouse 3T3 and rat PC-12 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-TrkA (rabbit polyclonal IgG) - DAM1413909 DAM1413909
      Anti-TrkA - 2382158 2382158
      Anti-TrkA - 2453236 2453236
      Anti-TrkA - 0605031286 0605031286
      Anti-TrkA - 0701050235 0701050235
      Anti-TrkA - 15499 15499
      Anti-TrkA - 18632 18632
      Anti-TrkA - 1955575 1955575
      Anti-TrkA - 1997723 1997723
      Anti-TrkA - 2056023 2056023

      References

      Reference overviewApplicationSpeciesPub Med ID
      The effect of antenatal depression and selective serotonin reuptake inhibitor treatment on nerve growth factor signaling in human placenta.
      Kaihola, H; Olivier, J; Poromaa, IS; Åkerud, H
      PloS one  10  e0116459  2015

      Show Abstract
      25611484 25611484
      Cis and trans RET signaling control the survival and central projection growth of rapidly adapting mechanoreceptors.
      Fleming, MS; Vysochan, A; Paixão, S; Niu, J; Klein, R; Savitt, JM; Luo, W
      eLife  4  e06828  2015

      Show Abstract
      25838128 25838128
      Cross-talk between androgen receptor/filamin A and TrkA regulates neurite outgrowth in PC12 cells.
      Di Donato, M; Bilancio, A; D'Amato, L; Claudiani, P; Oliviero, MA; Barone, MV; Auricchio, A; Appella, E; Migliaccio, A; Auricchio, F; Castoria, G
      Molecular biology of the cell  26  2858-72  2015

      Show Abstract
      26063730 26063730
      Boundary cap neural crest stem cells homotopically implanted to the injured dorsal root transitional zone give rise to different types of neurons and glia in adult rodents.
      Trolle, C; Konig, N; Abrahamsson, N; Vasylovska, S; Kozlova, EN
      BMC neuroscience  15  60  2014

      Show Abstract
      Immunohistochemistry24884373 24884373
      Sensory neurons do not induce motor neuron loss in a human stem cell model of spinal muscular atrophy.
      Schwab, AJ; Ebert, AD
      PloS one  9  e103112  2014

      Show Abstract
      25054590 25054590
      Coronin-1 is a neurotrophin endosomal effector that is required for developmental competition for survival.
      Suo, D; Park, J; Harrington, AW; Zweifel, LS; Mihalas, S; Deppmann, CD
      Nature neuroscience  17  36-45  2014

      Show Abstract
      24270184 24270184
      TAM receptors support neural stem cell survival, proliferation and neuronal differentiation.
      Ji, R; Meng, L; Jiang, X; Cvm, NK; Ding, J; Li, Q; Lu, Q
      PloS one  9  e115140  2014

      Show Abstract
      25514676 25514676
      Site-specific labeling of neurotrophins and their receptors via short and versatile peptide tags.
      Marchetti, L; De Nadai, T; Bonsignore, F; Calvello, M; Signore, G; Viegi, A; Beltram, F; Luin, S; Cattaneo, A
      PloS one  9  e113708  2014

      Show Abstract
      25426999 25426999
      An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
      Matusica, D; Skeldal, S; Sykes, AM; Palstra, N; Sharma, A; Coulson, EJ
      The Journal of biological chemistry  288  11144-54  2013

      Show Abstract
      Western Blotting23471969 23471969
      Establishment of neurovascular congruency in the mouse whisker system by an independent patterning mechanism.
      Oh, WJ; Gu, C
      Neuron  80  458-69  2013

      Show Abstract
      Immunohistochemistry24139045 24139045

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies