05-829 | Anti-α-Tubulin Antibody, clone DM1A

05-829
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, Po, B, Av, Gp, HICC, IF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-829
      Replaces04-1117
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-α-Tubulin Antibody, clone DM1A
      Alternate Names
      • Alpha-tubulin 3
      • Tubulin B-alpha-1
      • Tubulin alpha-3 chain
      • tubulin, alpha 1a
      • tubulin, alpha 3
      • tubulin, alpha, brain-specific
      Background InformationMicrotubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
      References
      Product Information
      FormatPurified
      Control
      • A431 cell lysate.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide and 30% glycerol.
      Applications
      ApplicationAnti-α-Tubulin Antibody, clone DM1A is an antibody against α-Tubulin for use in IC, IF & WB.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunofluorescence:
      (Breitling, F., 1986)
      Biological Information
      ImmunogenPurified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).
      Epitopea.a. 426-450
      CloneDM1A
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes α-Tubulin, clone DM1A, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to amino acids 426-450.
      IsotypeIgG1
      Species Reactivity
      • Rat
      • Mouse
      • Pig
      • Bovine
      • Avian
      • Guinea Pig
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMicrotubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
      Gene Symbol
      • TUBA1A
      • hum-a-tub2
      • FLJ25113
      • B-ALPHA-1
      • TUBA3
      • hum-a-tub1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q71U36 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 451 amino acids; 50136 Da
      SUBUNIT: Dimer of alpha and beta chains.
      PTM: Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively (By similarity).
      SIMILARITY: SwissProt: Q71U36 ## Belongs to the tubulin family.
      Molecular Weight50-60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on non-stimulated A431 lysate.

      Western Blot Analysis:
      0.1-1 µg/mL of this lot detected α- Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-#945;-Tubulin, clone DM1A - 2144401 2144401
      Anti-#945;-Tubulin, clone DM1A - 2016030 2016030
      Anti--Tubulin, clone DM1A - 2056018 2056018
      Anti--Tubulin, clone DM1A - 2106959 2106959
      Anti--Tubulin, clone DM1A - 2193150 2193150
      Anti--Tubulin, clone DM1A - 2225234 2225234
      Anti--Tubulin, clone DM1A - 2281661 2281661
      Anti--Tubulin, clone DM1A - DAM1687519 DAM1687519
      Anti--Tubulin, clone DM1A - DAM1724029 DAM1724029
      Anti--Tubulin, clone DM1A - DAM1764404 DAM1764404

      References

      Reference overviewApplicationSpeciesPub Med ID
      Inhibition of Translation Initiation by Protein 169: A Vaccinia Virus Strategy to Suppress Innate and Adaptive Immunity and Alter Virus Virulence.
      Strnadova, P; Ren, H; Valentine, R; Mazzon, M; Sweeney, TR; Brierley, I; Smith, GL
      PLoS pathogens  11  e1005151  2015

      Show Abstract
      26334635 26334635
      Tumor-derived microvesicles mediate human breast cancer invasion through differentially glycosylated EMMPRIN.
      Menck, K; Scharf, C; Bleckmann, A; Dyck, L; Rost, U; Wenzel, D; Dhople, VM; Siam, L; Pukrop, T; Binder, C; Klemm, F
      Journal of molecular cell biology  7  143-53  2015

      Show Abstract
      25503107 25503107
      Golgi anti-apoptotic proteins are highly conserved ion channels that affect apoptosis and cell migration.
      Carrara, G; Saraiva, N; Parsons, M; Byrne, B; Prole, DL; Taylor, CW; Smith, GL
      The Journal of biological chemistry  290  11785-801  2015

      Show Abstract
      25713081 25713081
      Programmed cell death 5 mediates HDAC3 decay to promote genotoxic stress response.
      Choi, HK; Choi, Y; Park, ES; Park, SY; Lee, SH; Seo, J; Jeong, MH; Jeong, JW; Jeong, JH; Lee, PC; Choi, KC; Yoon, HG
      Nature communications  6  7390  2015

      Show Abstract
      26077467 26077467
      Prostaglandin E2 promotes MYCN non-amplified neuroblastoma cell survival via β-catenin stabilization.
      Jansen, SR; Holman, R; Hedemann, I; Frankes, E; Elzinga, CR; Timens, W; Gosens, R; de Bont, ES; Schmidt, M
      Journal of cellular and molecular medicine  19  210-26  2015

      Show Abstract
      Western BlottingHuman25266063 25266063
      IκBβ-mediated NF-κB activation confers protection against hyperoxic lung injury.
      Michaelis, KA; Agboke, F; Liu, T; Han, K; Muthu, M; Galambos, C; Yang, G; Dennery, PA; Wright, CJ
      American journal of respiratory cell and molecular biology  50  429-38  2014

      Show Abstract
      Western Blotting24066808 24066808
      Mitochondrial topoisomerase I (top1mt) is a novel limiting factor of doxorubicin cardiotoxicity.
      Khiati, S; Dalla Rosa, I; Sourbier, C; Ma, X; Rao, VA; Neckers, LM; Zhang, H; Pommier, Y
      Clinical cancer research : an official journal of the American Association for Cancer Research  20  4873-81  2014

      Show Abstract
      24714774 24714774
      MicroRNA-7 inhibits multiple oncogenic pathways to suppress HER2Δ16 mediated breast tumorigenesis and reverse trastuzumab resistance.
      Huynh, FC; Jones, FE
      PloS one  9  e114419  2014

      Show Abstract
      Western Blotting25532106 25532106
      HER4 selectively coregulates estrogen stimulated genes associated with breast tumor cell proliferation.
      Han, W; Jones, FE
      Biochemical and biophysical research communications  443  458-63  2014

      Show Abstract
      Western Blotting24333426 24333426
      In vivo transcriptional governance of hair follicle stem cells by canonical Wnt regulators.
      Lien, WH; Polak, L; Lin, M; Lay, K; Zheng, D; Fuchs, E
      Nature cell biology  16  179-90  2014

      Show Abstract
      24463605 24463605

      Brochure

      Title
      AXIS: Axon Investigation System

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      Alternative Format

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies