05-647 | Anti-Tyrosinase Antibody, clone T311

05-647
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-647
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Tyrosinase Antibody, clone T311
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-Tyrosinase Antibody, clone T311 is validated for use in WB, IH for the detection of Tyrosinase.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesNot recommended for Immunoprecipitation
      Biological Information
      ImmunogenPurified recombinant Tyrosinase corresponding to residues 5-456 of human Tyrosinase
      CloneT311
      HostMouse
      SpecificityTyrosinase
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TYR
      • LB24-AB
      • tyrosinase
      • OCAIA
      • SK29-AB
      • OCA1A
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14679 # This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
      COFACTOR: Binds 2 copper ions per subunit.
      SIZE: 529 amino acids; 60393 Da
      SUBCELLULAR LOCATION: Melanosome membrane; Single-pass type I membrane protein.
      DISEASE: SwissProt: P14679 # Defects in TYR are the cause of oculocutaneous albinism type IA (OCA-IA) [MIM:203100]. OCA-I, also known as tyrosinase negative oculocutaneous albinism, is an autosomal recessive disorder characterized by absence of pigment in hair, skin and eyes. OCA-I is divided into 2 types: type IA, characterized by complete lack of tyrosinase activity due to production of an inactive enzyme, and type IB characterized by reduced activity of tyrosinase. OCA-IA patients presents with the life-long absence of melanin pigment after birth and manifest increased sensitivity to ultraviolet radiation and to predisposition to skin cancer. & Defects in TYR are the cause of oculocutaneous albinism type IB (OCA-IB) [MIM:606952]; also known as albinism yellow mutant type. OCA-IB patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal- to-moderate amounts of cutaneous and ocular pigment. & Defects in TYR are the cause of oculocutaneous albinism type I temperature-sensitive (OCA-ITS) [MIM:606952]. OCA-ITS patients have white axillary and scalp hair and pigmented arm and leg hair.
      SIMILARITY: SwissProt: P14679 ## Belongs to the tyrosinase family.
      Molecular Weight70-80kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from SK-MEL-19 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Tyrosinase, clone T311 (mouse monoclonal IgG2a) 2922056
      Anti-Tyrosinase, clone T311 - 22183 22183
      Anti-Tyrosinase, clone T311 -2779124 2779124

      References

      Reference overviewApplicationPub Med ID
      Structure-toxicity relationship of phenolic analogs as anti-melanoma agents: an enzyme directed prodrug approach.
      Vad NM, Kandala PK, Srivastava SK, Moridani MY
      Chemico-biological interactions  183  462-71  2010

      Show Abstract Full Text Article
      19944085 19944085
      Biochemical mechanism of caffeic acid phenylethyl ester (CAPE) selective toxicity towards melanoma cell lines.
      Shashi K Kudugunti,Nikhil M Vad,Amanda J Whiteside,Bhakti U Naik,Mohd A Yusuf,Kalkunte S Srivenugopal,Majid Y Moridani
      Chemico-biological interactions  188  2010

      Show Abstract Full Text Article
      20685355 20685355
      Efficacy of acetaminophen in skin B16-F0 melanoma tumor-bearing C57BL/6 mice.
      Nikhil M Vad,Shashi K Kudugunti,Daniel Graber,Nathan Bailey,Kalkunte Srivenugopal,Majid Y Moridani
      International journal of oncology  35  2009

      Show Abstract
      19513568 19513568
      T311--an anti-tyrosinase monoclonal antibody for the detection of melanocytic lesions in paraffin embedded tissues.
      Jungbluth, A A, et al.
      Pathol. Res. Pract., 196: 235-42 (2000)  2000

      Show Abstract
      Immunohistochemistry (Tissue)10782467 10782467
      Immunophenotyping of melanomas for tyrosinase: implications for vaccine development
      Chen, Y. T., et al
      Proc Natl Acad Sci U S A, 92:8125-9 (1995)  1995

      Immunoblotting (Western), Enzyme Immunoassay (ELISA)7667256 7667256