05-253MG | Anti-ZAP-70 Antibody, clone 2F3.2

05-253MG
1 mg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-253MG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-ZAP-70 Antibody, clone 2F3.2
      OverviewNote: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.
      References
      Product Information
      FormatPurified
      PresentationPBS
      Applications
      ApplicationDetect ZAP-70 using this Anti-ZAP-70 Antibody, clone 2F3.2 validated for use in FC, IH, IP & WB.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesFor testing in IHC, please see the following link (PDF): http://www.propathlab.com/pdf/focus_dec_2003.pdf
      Biological Information
      ImmunogenGST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254
      Cloneclone 2F3.2
      HostMouse
      Specificityhuman ZAP-70, does not recognize murine ZAP-70
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
      Gene Symbol
      • ZAP70
      • ZAP-70
      • TZK
      • STD
      • SRK
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
      SIZE: 619 amino acids; 69872 Da
      SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
      TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
      DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
      PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
      DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
      Molecular Weight70kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated on RIPA lysates prepared from Jurkat cells or Hut-78 cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ZAP-70 (human), clone 2F3.2 (mouse monoclonal IgG2a) 2891854
      Anti-ZAP-70 (human), clone 2F3.2 - JBC1356674 JBC1356674
      Anti-ZAP-70 (human), clone 2F3.2 -2595016 2595016
      Anti-ZAP-70, clone 2F3.2 - 24680 24680
      Anti-ZAP-70, clone 2F3.2 - 26514A 26514A

      References

      Reference overviewApplicationPub Med ID
      TCR signaling: another Abl-bodied kinase joins the cascade.
      Wange, Ronald L
      Curr. Biol., 14: R562-4 (2004)  2004

      Show Abstract
      15268875 15268875
      ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia.
      Crespo, Marta, et al.
      N. Engl. J. Med., 348: 1764-75 (2003)  2003

      Show Abstract
      Immunoblotting (Western)12724482 12724482
      The Syk family of protein tyrosine kinases in T-cell activation and development.
      Chu, D H, et al.
      Immunol. Rev., 165: 167-80 (1998)  1998

      9850860 9850860

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies