06-1352 | Anti-acetyl Histone H4 (Lys12) Antibody

06-1352
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, DrWB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue Number06-1352
      DescriptionAnti-acetyl Histone H4 (Lys12) Antibody
      Alternate Names
      • H4K12Ac
      • Histone H4 (acetyl K12)
      • H4 histone family, member A
      • histone 1, H4a
      • histone cluster 1, H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatSerum
      Control
      • Sodium butyrate treated and untreated HeLa cell lysate
      PresentationUnpurified rabbit polyclonal in serum with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-acetyl Histone H4 (Lys12) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect acetyl Histone H4 (Lys12) also known as H4K12Ac, Histone H4 (acetyl K12).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H4 in HeLa and A431 cells.
      Biological Information
      ImmunogenLinear peptide corresponding to Arabidopsis thaliana Histone H4 acetylated at and around acetylated Lys12.
      EpitopeLys12
      HostRabbit
      SpecificityThis antibody recognizes Histone H4 acetylated in and around Lys12.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Drosophila
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with Arabidopsis thaliana (higher plant) and Drosophila based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus By similarity.

      SEQUENCE SIMILARITIES: Belongs to the histone H4 family.
      Molecular Weight~ 14 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in sodium butyrate treated and untreated HeLa cell lysate.

      Western Blot Analysis: 1:2,000 dilution of this antibody detected Histone H4 on 10 µg of sodium butyrate treated and untreated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl Histone H4 (Lys12) - 2036703 2036703
      Anti-acetyl Histone H4 (Lys12) - NG1713104 NG1713104
      Anti-acetyl Histone H4 (Lys12) - NG1809738 NG1809738
      Anti-acetyl Histone H4 (Lys12) - NG1853785 NG1853785
      Anti-acetyl Histone H4 (Lys12) - NG1894430 NG1894430
      Anti-acetyl Histone H4 (Lys12) - NRG1685072 NRG1685072

      References

      Reference overviewPub Med ID
      Histone deacetylase expression in white matter oligodendrocytes after stroke.
      Kassis, H; Chopp, M; Liu, XS; Shehadah, A; Roberts, C; Zhang, ZG
      Neurochemistry international  77  17-23  2014

      Show Abstract
      24657831 24657831
      Essential role of DPPA3 for chromatin condensation in mouse oocytogenesis.
      Liu, YJ; Nakamura, T; Nakano, T
      Biology of reproduction  86  40  2012

      Show Abstract
      22034526 22034526
      Impaired coenzyme A synthesis in fission yeast causes defective mitosis, quiescence-exit failure, histone hypoacetylation and fragile DNA.
      Nakamura, T; Pluskal, T; Nakaseko, Y; Yanagida, M
      Open biology  2  120117  2012

      Show Abstract
      23091701 23091701