05-185 | Anti-avian Src Antibody, clone EC10

05-185
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AvIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-185
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-avian Src Antibody, clone EC10
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, with 0.05% sodium azide
      Applications
      ApplicationAnti-avian Src Antibody, clone EC10 detects level of avian Src & has been published & validated for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenRecombinant chicken pp60src
      Cloneclone EC10
      HostMouse
      Specificityspecific only for avian Src; this antibody is excellent for distinguishing pp60src in cells transfected with v-src or c-src expression vectors.
      IsotypeIgG2b
      Species Reactivity
      • Avian
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • SRC
      • c-src
      • p60-Src
      • SRC1
      • c-SRC
      • ASV
      • pp60c-src
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummarySIZE: 536 amino acids; 59835 Da
      SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
      PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
      SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated in (60kDa) on a RIPA lysate of chicken embryo fibroblasts (CEF)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RNA polymerase II, clone CTD4H8 -2705647 2705647
      Anti-avian Src, clone EC10 (mouse monoclonal IgG2b) - 2138004 2138004
      Anti-avian Src, clone EC10 (mouse monoclonal IgG2b) 2942429
      Anti-avian Src, clone EC10 (mouse monoclonal IgG2b) -2488970 2488970
      Anti-avian Src, clone EC10 - 16381 16381
      Anti-avian Src, clone EC10 - 19616 19616
      Anti-avian Src, clone EC10 - 23598 23598
      Anti-avian Src, clone EC10 - 2894141 2894141
      Anti-avian Src, clone EC10 - JBC1381915 JBC1381915
      Anti-avian Src, clone EC10 -2709107 2709107

      References

      Reference overviewApplicationPub Med ID
      Doubles game: Src-Stat3 versus p53-PTEN in cellular migration and invasion.
      Mukhopadhyay, UK; Mooney, P; Jia, L; Eves, R; Raptis, L; Mak, AS
      Molecular and cellular biology  30  4980-95  2010

      Show Abstract Full Text Article
      20733006 20733006
      SRC induces podoplanin expression to promote cell migration.
      Shen, Y; Chen, CS; Ichikawa, H; Goldberg, GS
      The Journal of biological chemistry  285  9649-56  2010

      Show Abstract
      Western Blotting20123990 20123990
      Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.
      Töyli, M; Rosberg-Kulha, L; Capra, J; Vuoristo, J; Eskelinen, S
      Laboratory investigation; a journal of technical methods and pathology  90  915-28  2010

      Show Abstract
      20212454 20212454
      p53 suppresses Src-induced podosome and rosette formation and cellular invasiveness through the upregulation of caldesmon.
      Utpal K Mukhopadhyay,Robert Eves,Lilly Jia,Patrick Mooney,Alan S Mak
      Molecular and cellular biology  29  2009

      Show Abstract Full Text Article
      19349302 19349302
      Coordinate suppression of Sdpr and Fhl1 expression in tumors of the breast, kidney, and prostate.
      Xun Li,Zhenyu Jia,Yongquan Shen,Hitoshi Ichikawa,Jonathan Jarvik,Robert G Nagele,Gary S Goldberg
      Cancer science  99  2008

      Show Abstract
      18422756 18422756
      SRC utilizes Cas to block gap junctional communication mediated by connexin43.
      Shen, Y; Khusial, PR; Li, X; Ichikawa, H; Moreno, AP; Goldberg, GS
      The Journal of biological chemistry  282  18914-21  2007

      Show Abstract
      Western Blotting17488714 17488714
      Normal cells control the growth of neighboring transformed cells independent of gap junctional communication and SRC activity.
      David B Alexander, Hitoshi Ichikawa, John F Bechberger, Virginijus Valiunas, Misao Ohki, Christian C G Naus, Takehiko Kunimoto, Hiroyuki Tsuda, W Todd Miller, Gary S Goldberg
      Cancer research  64  1347-58  2004

      Show Abstract
      14973064 14973064
      Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells.
      Goldberg, GS; Alexander, DB; Pellicena, P; Zhang, ZY; Tsuda, H; Miller, WT
      The Journal of biological chemistry  278  46533-40  2003

      Show Abstract Full Text Article
      12972425 12972425
      Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B
      Luban, J., et al
      Cell, 73:1067-78 (1993)  1993

      Immunoblotting (Western)8513493 8513493
      Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases.
      Kanner, S B, et al.
      Proc. Natl. Acad. Sci. U.S.A., 87: 3328-32 (1990)  1990

      Show Abstract
      2110361 2110361

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