07-1800 | Anti-eIF4G Antibody

07-1800
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IFRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1800
      DescriptionAnti-eIF4G Antibody
      Alternate Names
      • eIF-4-gamma 1
      • eIF-4G 1
      • eukaryotic translation initiation factor 4 gamma 1
      Background InformationeIF4F is a translation initiation factor containing three subunits: eIF4A, eIF4E, and eIF4G. This complex is a key component in promoting ribosomal binding to the mRNA. eIF4G interacts with both eIF4A and eIF4E though its carboxy and amino terminal domains, respectively. eIF4E binds to the cap structure of the mRNA, while the ATP-dependent RNA helicase eIF4A unwinds the secondary structure of the 5’ UTR. eIF4G plays a critical role in coordinating the assembly of the eukaryotic initiation factors. It acts as a bridge to bind the cap-bound eIF4E, eIF4AI&II, the eIF3 complex, PABP1, and the kinase Mnk1 (Lejeune, F., et al., 2004) in a large eukaryotic initiation factor complex
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% NaN3.
      Applications
      ApplicationAnti-eIF4G Antibody is an antibody against eIF4G for use in WB & IF.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Biological Information
      ImmunogenSynthetic peptide corresponding to N-terminal region of human eIF4G.
      EpitopeN-terminus
      HostRabbit
      SpecificityThis antibody detects eIF4G at its N-terminal region.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and Mouse. Not tested on other species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a component of the protein complex EIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure, and recruitment of mRNA to the ribosome. Alternative splicing results in five transcript variants encoding four distinct isoforms. [provided by RefSeq]
      Gene Symbol
      • EIF4-gamma
      • EIF4F
      • EIF4G
      • EIF4GI
      • eIF-4G1
      • p220
      UniProt Number
      UniProt SummaryFUNCTION: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
      SUBUNIT STRUCTURE: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis.
      PTM: Phosphorylated at multiple sites in vivo.
      Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
      SEQUENCE SIMILARITIES: Belongs to the eIF4G family.
      Contains 1 MI domain.
      Contains 1 MIF4G domain.
      Contains 1 W2 domain.
      SEQUENCE CAUTION: The sequence BAA02185.1 differs from that shown. Reason: Frameshift at several positions.
      Molecular Weight220 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on HeLa cytoplasmic extract.

      Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect eIF4G in HeLa cytoplasmic extract and lysates from 293T and S10 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications