05-1516 | Anti-hnRNP U Antibody, clone 3G6

05-1516
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ICC, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1516
      DescriptionAnti-hnRNP U Antibody, clone 3G6
      Alternate Names
      • Scaffold attachment factor A
      • heterogeneous nuclear ribonucleoprotein U
      • heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
      • hnRNP U
      • hnRNP U protein
      • p120 nuclear protein
      Background InformationHeterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. However, its role in the regulation of gene expression is as yet poorly understood. Proteins of the heterogeneous nuclear ribonucleoparticles (hnRNP) family form a structurally diverse group of RNA binding proteins implicated in various functions. Recently, hnRNP proteins have been shown to hinder communication between factors bound to different splice sites. Conversely, several reports have described a positive role for some hnRNP proteins in pre-mRNA splicing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements.
      References
      Product Information
      FormatPurified
      Control
      • K562 cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-hnRNP U Antibody, clone 3G6 (mouse monoclonal antibody) validated in WB, IP, ICC, ELISA to detect hnRNP U also known as heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • ELISA
      Application NotesImmunoprecipitation
      Reported by external laboratory using a represenative lot of this antibody
      Immunocytochemistry
      Reported by external laboratory using a represenative lot of this antibody
      ELISA
      Reported by external laboratory using a represenative lot of this antibody
      Biological Information
      ImmunogenRecombinant protein corresponding to human hnRNP U.
      EpitopeUnknown
      Clone3G6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes hnRNP U.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq].
      Gene Symbol
      • hnrnpu
      • HNRPU
      • SAF-A
      • SAFA
      • U21.1
      • hnRNPU
      • p120
      • pp120
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Bind to double- and single-stranded DNA and RNA.

      SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1. Ligand for CR2.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cell surface. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface.

      PTM: Extensively phosphorylated.

      Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine.

      SEQUENCE SIMILARITIES: Contains 1 B30.2/SPRY domain.

      Contains 1 SAP domain.

      SEQUENCE CAUTION: The sequence AAC19382.1 differs from that shown. Reason: Miscellaneous discrepancy. Aberrant splicing.
      Molecular WeightCalculated molecular weight is 90 kDa. However protein is usually observed at ~120 kDa. (Dreyfuss G, Kim VN, Kataoka N. Nat Rev Mol Cell Biol. 2002 Mar;3(3):195-205.)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

      Western Blot Analysis: 0.01 µg/ml of this antibody detected hnRNP U on 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-hnRNP U, clone 3G6 2842912
      Anti-hnRNP U, clone 3G6 - 2064317 2064317
      Anti-hnRNP U, clone 3G6 - 2360022 2360022
      Anti-hnRNP U, clone 3G6 - NG1827442 NG1827442
      Anti-hnRNP U, clone 3G6 - NG1931392 NG1931392
      Anti-hnRNP U, clone 3G6 - NRG1687967 NRG1687967
      Anti-hnRNP U, clone 3G6 -2521176 2521176
      Anti-hnRNP U, clone 3G6 -2603874 2603874
      Anti-hnRNP U, clone 3G6 -2608746 2608746
      Anti-hnRNP U, clone 3G6 -2736472 2736472

      References

      Reference overviewPub Med ID
      Relationship of one form of human histamine-releasing factor to connective tissue activating peptide-III.
      M L Baeza,S R Reddigari,D Kornfeld,N Ramani,E M Smith,P A Hossler,T Fischer,C W Castor,P G Gorevic,A P Kaplan
      The Journal of clinical investigation  85  1990

      Show Abstract Full Text Article
      1692035 1692035

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies