06-573 | Anti-iNOS/NOS II Antibody, NT

06-573
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, B, Sh, GpIHC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-573
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-iNOS/NOS II Antibody, NT
      OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
      Alternate Names
      • Hepatocyte NOS 3
      • Inducible NO synthase 3
      • Inducible NOS 3
      • NOS type II 3
      • NOS, type II 2
      • nitric oxide synthase 2, inducible
      • nitric oxide synthase 2A
      • nitric oxide synthase 2A (inducible, hepatocytes)
      • nitric oxide synthase, macrophage 2
      Background InformationNitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
      References
      Product Information
      FormatPurified
      Control
      • INFγ and LPS treated L929B cell lysate, cytokine stimulated A549 cells, cytokine stimulated rat fibroblasts
      PresentationPurified IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. Frozen solution.
      Applications
      ApplicationThis Anti-iNOS/NOS II Antibody, NT is validated for use in IH, IP, WB for the detection of iNOS/NOS II.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody has been reported to detect iNOS/NOS II in 2% paraformaldehyde-fixed sheep lung (Sherman, T.S., 1999).

      Immunoprecipitation:
      4 μg of a previous lot immunoprecipitated iNOS/NOS II from 500 μg of L929B cells which had been stimulated with 10 ng/mL IFNg and 1 μg/mL LPS.
      Biological Information
      ImmunogenPartial fusion protein raised against the N-terminus of murine iNOS/NOS II.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes N-terminus of iNOS, Mr 125 kDa. Does not cross-react with nNOS/NOS I.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Bovine
      • Sheep
      • Guinea Pig
      Species Reactivity NoteMouse, bovine, sheep and guinea pig. Other species cross-reactivity is unknown.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
      Gene Symbol
      • EC 1.14.13.39
      • HEP-NOS
      • INOS
      • NOS
      • NOS2A
      • iNOS
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
      COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
      SIZE: 1153 amino acids; 131117 Da
      SUBUNIT: Homodimer. Binds SLC9A3R1.
      TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
      SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Molecular Weight125 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on lysates from L929B cells that had been stimulated IFNγ and LPS.

      Western Blot Analysis: 1-4 μg/mL of this lot detected iNOS/NOS II in RIPA lysates from RAW 264.7 cells that had been stimulated with LPS. 2 μg/mL of a previous lot detected iNOS/NOS II from similarly treated L8 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-iNOS/NOS II (rabbit polyclonal IgG) - DAM1408770 DAM1408770
      Anti-iNOS/NOS II, NT - 2135181 2135181
      Anti-iNOS/NOS II, NT - 15441 15441
      Anti-iNOS/NOS II, NT - 2019588 2019588
      Anti-iNOS/NOS II, NT - 2055512 2055512
      Anti-iNOS/NOS II, NT - 21421 21421
      Anti-iNOS/NOS II, NT - 2199772 2199772
      Anti-iNOS/NOS II, NT - 24123 24123
      Anti-iNOS/NOS II, NT - 27365 27365
      Anti-iNOS/NOS II, NT - DAM1604964 DAM1604964

      References

      Reference overviewApplicationPub Med ID
      Inflammatory stimuli induce inhibitory S-nitrosylation of the deacetylase SIRT1 to increase acetylation and activation of p53 and p65.
      Shinozaki, S; Chang, K; Sakai, M; Shimizu, N; Yamada, M; Tanaka, T; Nakazawa, H; Ichinose, F; Yamada, Y; Ishigami, A; Ito, H; Ouchi, Y; Starr, ME; Saito, H; Shimokado, K; Stamler, JS; Kaneki, M
      Science signaling  7  ra106  2014

      Show Abstract
      25389371 25389371
      Prolactin promotes cartilage survival and attenuates inflammation in inflammatory arthritis.
      Adán, N; Guzmán-Morales, J; Ledesma-Colunga, MG; Perales-Canales, SI; Quintanar-Stéphano, A; López-Barrera, F; Méndez, I; Moreno-Carranza, B; Triebel, J; Binart, N; Martínez de la Escalera, G; Thebault, S; Clapp, C
      The Journal of clinical investigation  123  3902-13  2013

      Show Abstract
      23908112 23908112
      Extrathymically generated regulatory T cells control mucosal TH2 inflammation.
      Josefowicz, SZ; Niec, RE; Kim, HY; Treuting, P; Chinen, T; Zheng, Y; Umetsu, DT; Rudensky, AY
      Nature  482  395-9  2012

      Show Abstract
      22318520 22318520
      Arginase II Promotes Macrophage Inflammatory Responses Through Mitochondrial Reactive Oxygen Species, Contributing to Insulin Resistance and Atherogenesis.
      Ming, XF; Rajapakse, AG; Yepuri, G; Xiong, Y; Carvas, JM; Ruffieux, J; Scerri, I; Wu, Z; Popp, K; Li, J; Sartori, C; Scherrer, U; Kwak, BR; Montani, JP; Yang, Z
      Journal of the American Heart Association  1  e000992  2012

      Show Abstract
      23130157 23130157
      Expression and cellular localization of inducible nitric oxide synthase in lipopolysaccharide-treated rat kidneys.
      Choi, JY; Nam, SA; Jin, DC; Kim, J; Cha, JH
      The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society  60  301-15  2012

      Show Abstract
      22260992 22260992
      Deficits in spatial learning and memory is associated with hippocampal volume loss in aged apolipoprotein E4 mice.
      Jun-xiang Yin,Gregory H Turner,Hao-jie Lin,Stephen W Coons,Jiong Shi
      Journal of Alzheimer's disease : JAD  27  2011

      Show Abstract
      21743131 21743131
      The EBV BZLF1 protein inhibits TNFR1 expression through effects on cellular C/EBP proteins.
      Bristol JA, Robinson AR, Barlow EA, Kenney SC
      J Virol  2010

      Show Abstract
      20861254 20861254
      Inducible nitric oxide synthase is present in motor neuron mitochondria and Schwann cells and contributes to disease mechanisms in ALS mice.
      Kevin Chen,Frances J Northington,Lee J Martin
      Brain structure & function  214  2010

      Show Abstract Full Text Article
      19888600 19888600
      A critical role for CHIP in the aggresome pathway.
      Sha, Y; Pandit, L; Zeng, S; Eissa, NT
      Molecular and cellular biology  29  116-28  2009

      Show Abstract
      18955503 18955503
      Complications in the assignment of 14 and 28 Da mass shift detected by mass spectrometry as in vivo methylation from endogenous proteins.
      Sung Yun Jung,Yehua Li,Yi Wang,Yue Chen,Yingming Zhao,Jun Qin
      Analytical chemistry  80  2008

      Show Abstract Full Text Article
      18247584 18247584

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies