07-2276 | Anti-mSIN1 Antibody

07-2276
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2276
      DescriptionAnti-mSIN1 Antibody
      Alternate Names
      • MEKK2-interacting protein 1
      • Mitogen-activated protein kinase 2-associated protein 1
      • SAPK-interacting protein 1
      • Stress-activated map kinase-interacting protein 1
      • TORC2 subunit MAPKAP1
      • mitogen-activated protein kinase associated protein 1
      • ras inhibitor MGC2745
      • stress-activated map kinase interacting protein 1
      • stress-activated protein kinase-interacting 1
      Background InformationmSIN1 (mamalian stress-activated protein kinase (SAPK)-interacting protein 1, MAPKAP1) is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase along with SIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The complex that is activated primarily downstream of PI3 Kinase is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. SIN1 is also known to directly interact without proteins that include Ras, SPAKs, and JNK.
      References
      Product Information
      FormatAffinity Purified
      Control
      • C2C12 cell lysate
      PresentationPurified rabbit serum in PBS with 0.05% sodium azide.
      Applications
      ApplicationDetect the mSIN1 protein using this Anti-mSIN1 validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human mSIN1.
      EpitopeUnknown
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes mSIN1.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq]
      Gene Symbol
      • MAPKAP1
      • JC310
      • MIP1
      • SIN1
      • SIN1b
      • SIN1g
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.

      SUBUNIT STRUCTURE: Interacts with IFNAR2 By similarity. All isoforms except isoform 4 can be incorporated into the mammalian target of rapamycin complex 2 (mTORC2) which contains FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS.

      SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus.

      TISSUE SPECIFICITY: Ubiquitously expressed, with highest levels in heart and skeletal muscle.

      SEQUENCE SIMLARITIES: Belongs to the SIN1 family.
      Molecular WeightExpected:59 kDa, Observed 61 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in C2C12 cell lysate.

      Western Blot Analysis: A 1:1,000 of this antibody detected mSIN1 on 10 µg of C2C12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies