05-1564 | Anti-mTOR Antibody, clone 21A12.2

05-1564
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1564
      DescriptionAnti-mTOR Antibody, clone 21A12.2
      Alternate Names
      • TORC2-specific protein AVO3
      • rapamycin-insensitive companion of mTOR
      Background InformationmTOR (Mammalian Target of Rapamycin, FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 consists of at least mTOR, Raptor, and GL. mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The other mTOR complex, mTORC2, is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. TORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473. TORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
      References
      Product Information
      FormatPurified
      Control
      • HEK293 cell lysates
      PresentationPurified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% sodium azide.
      Applications
      ApplicationDetect mTOR using this Anti-mTOR Antibody, clone 21A12.2 validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesConfocal Immunofluorescent Immunocytochemistry:
      Biological Information
      ImmunogenFull-length human mTOR
      Cloneclone 21A12.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody only detects mTOR.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Other species to be tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DKFZp686B11164
      • KIAA1999
      • MGC39830
      • mAVO3
      • pianissimo
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Plays an essential role in embryonic growth and development By similarity. Part of the TORC2 complex which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.

      SUBUNIT STRUCTURE: Forms part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to FRAP1 and PROTOR1 within the TORC2 complex. May interact with PROTOR2.

      SEQUENCE CAUTION: The sequence CAH18135.1 differs from that shown. Reason: Frameshift at position 251.

      Molecular Weight~289 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting on HEK293 cell lysates.

      Western Blotting Analysis: 1:1,000 - 2,000 dilution of this antibody was used to detect mTOR in HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-mTOR, clone 21A12.2 2476749
      Anti-mTOR, clone 21A12.2 - 2376018 2376018
      Anti-mTOR, clone 21A12.2 - 2000824 2000824
      Anti-mTOR, clone 21A12.2 - 2278558 2278558
      Anti-mTOR, clone 21A12.2 - NG1607966 NG1607966
      Anti-mTOR, clone 21A12.2 - NG1688773 NG1688773
      Anti-mTOR, clone 21A12.2 - NG1825182 NG1825182
      Anti-mTOR, clone 21A12.2 - NG1917780 NG1917780
      Anti-mTOR, clone 21A12.2 -2623831 2623831
      Anti-mTOR, clone 21A12.2 -2686880 2686880

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies