07-450 | Anti-monomethyl-Histone H3 (Lys9) Antibody

07-450
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, ChDB, ICC, PIA, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-450
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-monomethyl-Histone H3 (Lys9) Antibody
      Alternate Names
      • H3K9me1
      • Histone H3 (mono methyl K9)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell extract
      PresentationPurified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
      Applications
      ApplicationAnti-monomethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 monomethylated on lysine 9 (H3K9me1). This purified antibody is specificity verified by dot blot (DB), published in peer reviewed journals, and has been validated in ICC, PIA & WB.
      Key Applications
      • Dot Blot
      • Immunocytochemistry
      • Peptide Inhibition Assay
      • Western Blotting
      Application NotesPeptide Competition:
      Specificity confirmed by the ability of 10 μM of the immunizing peptide to abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts (Figure B, Lane 2). No signal reductions were detected with preincubation with 10 μM di- or trimethyl-lysine 9 or di- or trimethyl-lysine 27 modified peptides. Some signal reduction detected after preincubation with 10 μM monomethyl-lysine 27 modified peptide (Figure B, Lane 5, page 2).

      Immunocytochemistry & Dot Blot: Reported by an independent laboratory1-4.
      Biological Information
      ImmunogenKLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKST… in which meK corresponds to monomethyl-lysine at residue 9 of human histone H3.
      EpitopeLys9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes monomethylated histone H3 (Lys9), Mr ~17 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Chicken
      Species Reactivity NoteHuman, mouse and chicken. Broad species cross-reactivity is expected based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      Modifications
      • Methylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3.

      Western Blot Analysis:
      0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells, but did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-monomethyl-Histone H3 (Lys9) - 1947729 1947729
      Anti-monomethyl-Histone H3 (Lys9) - 24441 24441
      Anti-monomethyl-Histone H3 (Lys9) - 2500469 2500469
      Anti-monomethyl-Histone H3 (Lys9) - 2986155 2986155
      Anti-monomethyl-Histone H3 (Lys9) - DAM1394811 DAM1394811
      Anti-monomethyl-Histone H3 (Lys9) - DAM1604974 DAM1604974
      Anti-monomethyl-Histone H3 (Lys9) - DAM1647876 DAM1647876
      Anti-monomethyl-Histone H3 (Lys9) - DAM1676177 DAM1676177
      Anti-monomethyl-Histone H3 (Lys9) - DAM1680820 DAM1680820
      Anti-monomethyl-Histone H3 (Lys9) - DAM1749937 DAM1749937

      References

      Reference overviewApplicationPub Med ID
      Methylation of histone H3 lysine 9 occurs during translation.
      Rivera, C; Saavedra, F; Alvarez, F; Díaz-Celis, C; Ugalde, V; Li, J; Forné, I; Gurard-Levin, ZA; Almouzni, G; Imhof, A; Loyola, A
      Nucleic acids research  43  9097-106  2015

      Show Abstract
      26405197 26405197
      Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis.
      Stroud, H; Do, T; Du, J; Zhong, X; Feng, S; Johnson, L; Patel, DJ; Jacobsen, SE
      Nature structural & molecular biology  21  64-72  2014

      Show Abstract
      24336224 24336224
      Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components.
      Alabert, C; Bukowski-Wills, JC; Lee, SB; Kustatscher, G; Nakamura, K; de Lima Alves, F; Menard, P; Mejlvang, J; Rappsilber, J; Groth, A
      Nature cell biology  16  281-93  2014

      Show Abstract
      24561620 24561620
      Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines.
      Maroschik, B; Gürtler, A; Krämer, A; Rößler, U; Gomolka, M; Hornhardt, S; Mörtl, S; Friedl, AA
      Radiation oncology (London, England)  9  15  2014

      Show Abstract
      Western Blotting24406105 24406105
      Stage-dependent and locus-specific role of histone demethylase Jumonji D3 (JMJD3) in the embryonic stages of lung development.
      Li, Q; Wang, HY; Chepelev, I; Zhu, Q; Wei, G; Zhao, K; Wang, RF
      PLoS genetics  10  e1004524  2014

      Show Abstract
      25079229 25079229
      Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T-cell differentiation.
      Li, Q; Zou, J; Wang, M; Ding, X; Chepelev, I; Zhou, X; Zhao, W; Wei, G; Cui, J; Zhao, K; Wang, HY; Wang, RF
      Nature communications  5  5780  2014

      Show Abstract
      Western Blotting25531312 25531312
      Epigenetic control of type 2 and 3 deiodinases in myogenesis: role of Lysine-specific Demethylase enzyme and FoxO3.
      Ambrosio, R; Damiano, V; Sibilio, A; De Stefano, MA; Avvedimento, VE; Salvatore, D; Dentice, M
      Nucleic acids research  41  3551-62  2013

      Show Abstract
      23396445 23396445
      Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment.
      Horrillo, A; Pezzolla, D; Fraga, MF; Aguilera, Y; Salguero-Aranda, C; Tejedo, JR; Martin, F; Bedoya, FJ; Soria, B; Hmadcha, A
      Cell death & disease  4  e570  2013

      Show Abstract
      23559004 23559004
      A replication-dependent passive mechanism modulates DNA demethylation in mouse primordial germ cells.
      Ohno, R; Nakayama, M; Naruse, C; Okashita, N; Takano, O; Tachibana, M; Asano, M; Saitou, M; Seki, Y
      Development (Cambridge, England)  140  2892-903  2013

      Show Abstract
      23760957 23760957
      Lysine-specific demethylase 1 (LSD1/KDM1A) contributes to colorectal tumorigenesis via activation of the Wnt/β-catenin pathway by down-regulating Dickkopf-1 (DKK1) [corrected].
      Huang, Z; Li, S; Song, W; Li, X; Li, Q; Zhang, Z; Han, Y; Zhang, X; Miao, S; Du, R; Wang, L
      PloS one  8  e70077  2013

      Show Abstract
      Western Blotting23922913 23922913

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      Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

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