05-1248 | Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306

05-1248
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIAMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1248
      DescriptionAnti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306
      Alternate Names
      • H3K9me1
      • Histone H3 (mono methyl K9)
      • H3-K9-HMTase 1
      • Histone H3-K9 methyltransferase 1
      • Lysine N-methyltransferase 1A
      • Position-effect variegation 3-9 homolog
      • Su(var)3-9 homolog 1
      • Suppressor of variegation 3-9 homolog 1
      • histone-lysine N-methyltransferase, H3 lysine-9 specific 1
      • suppressor of variegation 3-9 (Drosophila) homolog 1
      • suppressor of variegation 3-9 homolog 1 (Drosophila)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatPurified
      Control
      • Recombinant Histone H3 and HeLa acid extract.
      PresentationPurified mouse monoclonal IgG2a in PBS with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 (Mouse Monoclonal Antibody) validated in WB, PIA to detect monomethyl Histone H3 (Lys9) also known as H3K9me1.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesDot Blot Analysis: AbSurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 05-1248, Anti-monomethyl H3 (Lys9) at 2ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.

      Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides.
      Biological Information
      ImmunogenKLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3.
      Epitopemonomethylated H3 Lys9
      CloneCMA306
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1,with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • EC 2.1.1.43
      • KMT1A
      • MG44
      • OTTHUMP00000024298
      • SUV39H
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiaton, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length.
      CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Ref.7
      ENZYME REGULATION: Inhibited by S-adenosyl-L-homocysteine. Ref.20
      SUBUNIT STRUCTURE: Interacts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR. Ref.22 Ref.1 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.16 Ref.17 Ref.18 Ref.24 Ref.25
      SUBCELLULAR LOCATION: Nucleus. Centromere. Note: Associates with centromeric constitutive heterochromatin. Ref.6
      DEVELOPMENTAL STAGE: Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.
      DOMAIN: Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin. Ref.17
      PTM: Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function. Ref.6 Ref.21 Ref.26 Ref.27
      SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
      Contains 1 chromo domain.
      Contains 1 post-SET domain.
      Contains 1 pre-SET domain.
      Contains 1 SET domain.

      Molecular Weight~17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on recombinant Histone H3 and HeLa acid extract.
      Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-monomethyl Histone H3 (Lys9), -2743614 2743614
      Anti-monomethyl Histone H3 (Lys9), clone CMA306 - 2146003 2146003
      Anti-monomethyl Histone H3 (Lys9), clone CMA306 - 2299607 2299607
      Anti-monomethyl Histone H3 (Lys9), clone CMA306 - JBC1787345 JBC1787345
      Anti-monomethyl Histone H3 (Lys9), clone CMA306 - NG1710330 NG1710330
      Anti-monomethyl Histone H3 (Lys9), clone CMA306 - NG1830761 NG1830761
      Anti-monomethyl Histone H3 (Lys9), clone CMA306 - NG1905727 NG1905727
      Anti-monomethyl Histone H3 (Lys9), clone CMA306 - NRG1651104 NRG1651104
      Anti-monomethyl Histone H3 (Lys9), clone CMA306 -2607874 2607874

      References

      Reference overviewPub Med ID
      Jarid2 (Jumonji, AT rich interactive domain 2) regulates NOTCH1 expression via histone modification in the developing heart.
      Mysliwiec, MR; Carlson, CD; Tietjen, J; Hung, H; Ansari, AZ; Lee, Y
      The Journal of biological chemistry  287  1235-41  2012

      Show Abstract
      22110129 22110129

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      Life Science Research > Antibodies and Assays > Primary Antibodies