05-345 | Anti-p21/WAF1/Cip1 Antibody

05-345
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-345
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-p21/WAF1/Cip1 Antibody
      Alternate Names
      • CDK-interacting protein
      • CDK-interaction protein
      • DNA synthesis inhibitor
      • Melanoma differentiation-associated protein 6
      • cyclin-dependent kinase inhibitor 1A
      • melanoma differentiation associated protein 6
      • wild-type p53-activated fragment 1
      Background InformationTwo families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically. The biochemical activities and patterns of expression of CKIs during development implicate these proteins as the primary effectors of signaling pathways that control cell cycle exit, an event that is critical for differentiation. Studies have shown that p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is a relatively poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb, which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.
      References
      Product Information
      FormatPurified
      Control
      • Included Positive Antigen: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels.
      PresentationPurified, pooled monoclonal mouse IgGs in buffer containing 0.1 M Trisglycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationUse Anti-p21/WAF1/Cip1 Antibody (Mouse Monoclonal Antibody) validated in WB, IP to detect p21/WAF1/Cip1 also known as CDK-interacting protein or cyclin-dependent kinase inhibitor 1A.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation: 4 μg of a previous lot immunoprecipitated Cip1 from 500 μg of HeLa nuclear extract.
      Biological Information
      ImmunogenRecombinant full-length human Cip1 with a histidine tag.
      Epitopeamino acids 1-80
      CloneCP36, CP74
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Cip1, MW 21 kDa
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and mouse; other species cross-reactivity is unknown.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
      Gene Symbol
      • CDKN1A
      • MDA-6
      • SDI1
      • MDA6
      • p21
      • p21CIP1
      • P21
      • CIP1
      • PIC1
      • CDKN1
      • WAF1
      • CAP20
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P38936 # May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.
      SIZE: 164 amino acids; 18119 Da
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
      TISSUE SPECIFICITY: Expressed in all adult human tissues, with 5- fold lower levels observed in the brain.
      PTM: Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.
      SIMILARITY: SwissProt: P38936 ## Belongs to the CDI family.
      Molecular Weight21 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blotting on HeLa nuclear extract.

      Western Blot Analysis: 0.5-2 μg/mL of this antibody detected Cip1 in 20 μg of HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-p21/WAF1/Cip1 - 2113344 2113344
      Anti-p21/WAF1/Cip1 - 2153140 2153140
      Anti-p21/WAF1/Cip1 - 2382166 2382166
      Anti-p21/WAF1/Cip1 - 2436388 2436388
      Anti-p21/WAF1/Cip1 - 14519 14519
      Anti-p21/WAF1/Cip1 - 18834 18834
      Anti-p21/WAF1/Cip1 - 19329 19329
      Anti-p21/WAF1/Cip1 - 1969116 1969116
      Anti-p21/WAF1/Cip1 - 19727 19727
      Anti-p21/WAF1/Cip1 - 2019715 2019715

      References

      Reference overviewApplicationPub Med ID
      High levels of TopBP1 induce ATR-dependent shut-down of rRNA transcription and nucleolar segregation.
      Sokka, M; Rilla, K; Miinalainen, I; Pospiech, H; Syväoja, JE
      Nucleic acids research  43  4975-89  2015

      Show Abstract
      25916852 25916852
      Identification and characterization of nuclear and nucleolar localization signals in 58-kDa microspherule protein (MSP58).
      Yang, CP; Chiang, CW; Chen, CH; Lee, YC; Wu, MH; Tsou, YH; Yang, YS; Chang, WC; Lin, DY
      Journal of biomedical science  22  33  2015

      Show Abstract
      25981436 25981436
      Gene profiling characteristics of radioadaptive response in AG01522 normal human fibroblasts.
      Hou, J; Wang, F; Kong, P; Yu, PK; Wang, H; Han, W
      PloS one  10  e0123316  2015

      Show Abstract
      25886619 25886619
      The FGF-1-specific single-chain antibody scFv1C9 effectively inhibits breast cancer tumour growth and metastasis.
      Shi, H; Fu, C; Wang, W; Li, Y; Du, S; Cao, R; Chen, J; Sun, D; Zhang, Z; Wang, X; Zhu, X
      Journal of cellular and molecular medicine  18  2061-70  2014

      Show Abstract
      Western Blotting25124967 25124967
      The downregulation of GFI1 by the EZH2-NDY1/KDM2B-JARID2 axis and by human cytomegalovirus (HCMV) associated factors allows the activation of the HCMV major IE promoter and the transition to productive infection.
      Sourvinos, G; Morou, A; Sanidas, I; Codruta, I; Ezell, SA; Doxaki, C; Kampranis, SC; Kottakis, F; Tsichlis, PN
      PLoS pathogens  10  e1004136  2014

      Show Abstract
      Western Blotting24830456 24830456
      Effects of physiological levels of the green tea extract epigallocatechin-3-gallate on breast cancer cells.
      Zeng, L; Holly, JM; Perks, CM
      Frontiers in endocrinology  5  61  2014

      Show Abstract
      Western Blotting24847310 24847310
      Loss of compensatory pro-survival and anti-apoptotic modulator, IKKε, sensitizes ovarian cancer cells to CHEK1 loss through an increased level of p21.
      Kim, MK; Min, DJ; Wright, G; Goldlust, I; Annunziata, CM
      Oncotarget  5  12788-802  2014

      Show Abstract
      Western Blotting25474241 25474241
      The HDAC inhibitor LBH589 induces ERK-dependent prometaphase arrest in prostate cancer via HDAC6 inactivation and down-regulation.
      Chuang, MJ; Wu, ST; Tang, SH; Lai, XM; Lai, HC; Hsu, KH; Sun, KH; Sun, GH; Chang, SY; Yu, DS; Hsiao, PW; Huang, SM; Cha, TL
      PloS one  8  e73401  2013

      Show Abstract
      24023871 24023871
      Metformin suppresses growth of human head and neck squamous cell carcinoma via global inhibition of protein translation.
      Sikka, A; Kaur, M; Agarwal, C; Deep, G; Agarwal, R
      Cell cycle (Georgetown, Tex.)  11  1374-82  2012

      Show Abstract
      22421144 22421144
      Ablation of PRMT6 reveals a role as a negative transcriptional regulator of the p53 tumor suppressor.
      Neault, M; Mallette, FA; Vogel, G; Michaud-Levesque, J; Richard, S
      Nucleic acids research  40  9513-21  2012

      Show Abstract
      Western Blotting22904064 22904064

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      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies