05-655 | Anti-p21/WAF1/Cip1 Antibody, clone CP74

05-655
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-655
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-p21/WAF1/Cip1 Antibody, clone CP74
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-p21/WAF1/Cip1 Antibody, clone CP74 is a high quality Mouse Monoclonal Antibody for the detection of p21/WAF1/Cip1 & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant full-length human Cip1 with an N-terminal histidine tag; epitope corresponds to amino acids 1-80 of full-length human Cip1
      Cloneclone CP74
      HostMouse
      SpecificityCip1
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
      Gene Symbol
      • CDKN1A
      • MDA-6
      • SDI1
      • MDA6
      • p21
      • p21CIP1
      • PIC1
      • CIP1
      • P21
      • CDKN1
      • WAF1
      • CAP20
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P38936 # May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.
      SIZE: 164 amino acids; 18119 Da
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
      TISSUE SPECIFICITY: Expressed in all adult human tissues, with 5- fold lower levels observed in the brain.
      PTM: Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.
      SIMILARITY: SwissProt: P38936 ## Belongs to the CDI family.
      Molecular Weight21kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on nuclear extract from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-p21/WAF1/Cip1, clone CP74 (mouse monoclonal IgG2b#954;) - 2151078 2151078
      Anti-p21/WAF1/Cip1, clone CP74 (mouse monoclonal IgG2bK) 2945607
      Anti-p21/WAF1/Cip1, clone CP74 (mouse monoclonal IgG2bk) 2912590
      Anti-p21/WAF1/Cip1, clone CP74 (mouse monoclonal IgG2bk) - 1962658 1962658
      Anti-p21/WAF1/Cip1, clone CP74 (mouse monoclonal IgG2bk) - 2080300 2080300
      Anti-p21/WAF1/Cip1, clone CP74 (mouse monoclonal IgG2bk) - 2299569 2299569
      Anti-p21/WAF1/Cip1, clone CP74 (mouse monoclonal IgG2bk) - DAM1459197 DAM1459197
      Anti-p21/WAF1/Cip1, clone CP74 - 1350286 1350286
      Anti-p21/WAF1/Cip1, clone CP74 - 2037636 2037636
      Anti-p21/WAF1/Cip1, clone CP74 - 22208 22208

      References

      Reference overviewApplicationPub Med ID
      Phytometabolite Dehydroleucodine Induces Cell Cycle Arrest, Apoptosis, and DNA Damage in Human Astrocytoma Cells through p73/p53 Regulation.
      Bailon-Moscoso, N; González-Arévalo, G; Velásquez-Rojas, G; Malagon, O; Vidari, G; Zentella-Dehesa, A; Ratovitski, EA; Ostrosky-Wegman, P
      PloS one  10  e0136527  2015

      Show Abstract
      Western Blotting26309132 26309132
      The telomere deprotection response is functionally distinct from the genomic DNA damage response.
      Cesare, AJ; Hayashi, MT; Crabbe, L; Karlseder, J
      Molecular cell  51  141-55  2013

      Show Abstract
      23850488 23850488
      A chemical genomics screen to discover genes that modulate neural stem cell differentiation.
      Kim, KJ; Wang, J; Xu, X; Wu, S; Zhang, W; Qin, Z; Wu, F; Liu, A; Zhao, Y; Fang, H; Zhu, M; Zhao, J; Zhong, Z
      Journal of biomolecular screening  17  129-39  2012

      Show Abstract
      21948800 21948800
      Expression and function of EZH2 in synovial fibroblasts: epigenetic repression of the Wnt inhibitor SFRP1 in rheumatoid arthritis.
      Trenkmann M, Brock M, Gay RE, Kolling C, Speich R, Michel BA, Gay S, Huber LC
      Annals of the rheumatic diseases  70  1482-8. Epub 2011 Apr 22.  2011

      21515604 21515604
      Oncogenic functions of hMDMX in in vitro transformation of primary human fibroblasts and embryonic retinoblasts.
      Lenos, K; de Lange, J; Teunisse, AF; Lodder, K; Verlaan-de Vries, M; Wiercinska, E; van der Burg, MJ; Szuhai, K; Jochemsen, AG
      Molecular cancer  10  111  2011

      Show Abstract
      21910853 21910853
      N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) triggers MSH2 and Cdt2 protein-dependent degradation of the cell cycle and mismatch repair (MMR) inhibitor protein p21Waf1/Cip1.
      Jascur, T; Fotedar, R; Greene, S; Hotchkiss, E; Boland, CR
      The Journal of biological chemistry  286  29531-9  2011

      Show Abstract
      21725088 21725088
      Histone lysine methyltransferase Wolf-Hirschhorn syndrome candidate 1 is involved in human carcinogenesis through regulation of the Wnt pathway.
      Toyokawa, G; Cho, HS; Masuda, K; Yamane, Y; Yoshimatsu, M; Hayami, S; Takawa, M; Iwai, Y; Daigo, Y; Tsuchiya, E; Tsunoda, T; Field, HI; Kelly, JD; Neal, DE; Maehara, Y; Ponder, BA; Nakamura, Y; Hamamoto, R
      Neoplasia (New York, N.Y.)  13  887-98  2011

      Show Abstract Full Text Article
      22028615 22028615
      STX140 and STX641 cause apoptosis via the intrinsic mitochondrial pathway and down-regulate survivin and XIAP expression in ovarian and prostate cancer cells.
      Foster PA, Ho YT, Newman SP, Leese MP, Potter BV, Reed MJ, Purohit A
      Anticancer research  29  3751-7  2009

      Show Abstract
      19846905 19846905
      Heat shock protein 90 inhibitors attenuate LPS-induced endothelial hyperpermeability.
      Chatterjee, A; Snead, C; Yetik-Anacak, G; Antonova, G; Zeng, J; Catravas, JD
      American journal of physiology. Lung cellular and molecular physiology  294  L755-63  2008

      Show Abstract
      18245267 18245267
      The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo.
      Yang, Z Y, et al.
      Nat. Med., 1: 1052-6 (1995)  1995

      Show Abstract
      7489362 7489362

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      05-345 Anti-p21/WAF1/Cip1 Antibody Show Pricing & Availability
      MAB88058 Anti-p21waf1 Antibody, clone HZ52 Show Pricing & Availability
      MABE325 Anti-p21WAF1 Antibody, clone EA10 Show Pricing & Availability

      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies