07-476 | Anti-p75NTR (Neurotrophin Receptor) Antibody

07-476
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MIHC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-476
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-p75NTR (Neurotrophin Receptor) Antibody
      Alternate Names
      • CD271 antigen
      • Low affinity neurotrophin receptor p75NTR
      • Low-affinity nerve growth factor receptor
      • NGF receptor
      • low affinity nerve growth factor receptor
      • nerve growth factor receptor
      • nerve growth factor receptor (TNFR superfamily, member 16)
      • p75 ICD
      Background InformationThe Low Affinity Nerve Growth Factor Receptor (also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors which stimulate neuronal cells to survive and differentiate.
      The neurotrophins are comprised of four proteins, all of which bind to the LNGFR: Nerve Growth Factor (NGF), Brain Derived Neurotrophic Factor (BDNF), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4).
      Nerve growth factor, the prototypical growth factor, is a protein secreted by a neuron's target. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron. The NGF/TrkA complex is subsequently trafficked back to the cell body. This movement of NGF from axon tip to soma is thought to be involved in the long-distance signaling of neurons.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue.
      PresentationPurified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationDetect p75NTR (Neurotrophin Receptor) using this Anti-p75NTR (Neurotrophin Receptor) Antibody validated for use in IH, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      This antibody has been reported by an independent laboratory to immunoprecipitate p75 neurotrophin receptor.

      Immunohistochemistry:
      This antibody has been reported by an independent laboratory to detect p75 neurotrophin receptor in rat brain sections. (Roux, P., 1999)
      Biological Information
      ImmunogenGST fusion protein corresponding to the intracellular domain (residues 274-425) of rat p75 neurotrophin receptor.
      EpitopeIC domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes p75 neurotrophin receptor, MW ~75 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
      Gene Symbol
      • NGFR
      • Gp80-LNGFR
      • CD271
      • p75(NTR)
      • TNFRSF16
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
      SIZE: 427 amino acids; 45183 Da
      SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9.
      PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues.
      SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.
      Molecular Weight~75 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot in RIPA lysates from PC-12 cells.

      Western Blot Analysis:
      0.1-0.5 µg/mL of this antibody detected p75 neurotrophin receptor in RIPA lysates from PC-12 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-p75NTR (Neurotrophin Receptor) (rabbit polyclonal IgG) - 1966952 1966952
      Anti-p75NTR (Neurotrophin Receptor) - 2135184 2135184
      Anti-p75NTR (Neurotrophin Receptor) - 2388905 2388905
      Anti-p75NTR (Neurotrophin Receptor) - 2436416 2436416
      Anti-p75NTR (Neurotrophin Receptor) - 2019565 2019565
      Anti-p75NTR (Neurotrophin Receptor) - 2064520 2064520
      Anti-p75NTR (Neurotrophin Receptor) - 2243498 2243498
      Anti-p75NTR (Neurotrophin Receptor) - 2344667 2344667
      Anti-p75NTR (Neurotrophin Receptor) - 24530 24530
      Anti-p75NTR (Neurotrophin Receptor) - 32073 32073

      References

      Reference overviewApplicationPub Med ID
      Hair follicle bulge cultures yield class III β-tubulin-positive melanoglial cells.
      Locher, H; Saadah, N; de Groot, S; de Groot, JC; Frijns, JH; Huisman, MA
      Histochemistry and cell biology  144  87-91  2015

      Show Abstract
      Immunocytochemistry25724811 25724811
      p75(NTR)-dependent activation of NF-κB regulates microRNA-503 transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia.
      Caporali, A; Meloni, M; Nailor, A; Mitić, T; Shantikumar, S; Riu, F; Sala-Newby, GB; Rose, L; Besnier, M; Katare, R; Voellenkle, C; Verkade, P; Martelli, F; Madeddu, P; Emanueli, C
      Nature communications  6  8024  2015

      Show Abstract
      26268439 26268439
      Site-specific labeling of neurotrophins and their receptors via short and versatile peptide tags.
      Marchetti, L; De Nadai, T; Bonsignore, F; Calvello, M; Signore, G; Viegi, A; Beltram, F; Luin, S; Cattaneo, A
      PloS one  9  e113708  2014

      Show Abstract
      25426999 25426999
      Neurotrophins regulate ApoER2 proteolysis through activation of the Trk signaling pathway.
      Larios, JA; Jausoro, I; Benitez, ML; Bronfman, FC; Marzolo, MP
      BMC neuroscience  15  108  2014

      Show Abstract
      25233900 25233900
      Distribution and development of peripheral glial cells in the human fetal cochlea.
      Locher, H; de Groot, JC; van Iperen, L; Huisman, MA; Frijns, JH; Chuva de Sousa Lopes, SM
      PloS one  9  e88066  2014

      Show Abstract
      24498246 24498246
      Perturbation of Hoxb5 signaling in vagal and trunk neural crest cells causes apoptosis and neurocristopathies in mice.
      Kam, MK; Cheung, MC; Zhu, JJ; Cheng, WW; Sat, EW; Tam, PK; Lui, VC
      Cell death and differentiation  21  278-89  2014

      Show Abstract
      Immunofluorescence24141719 24141719
      Genetic rescue of Muenke syndrome model hearing loss reveals prolonged FGF-dependent plasticity in cochlear supporting cell fates.
      Mansour, SL; Li, C; Urness, LD
      Genes & development  27  2320-31  2013

      Show Abstract
      Immunohistochemistry24145799 24145799
      An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
      Matusica, D; Skeldal, S; Sykes, AM; Palstra, N; Sharma, A; Coulson, EJ
      The Journal of biological chemistry  288  11144-54  2013

      Show Abstract
      Western Blotting23471969 23471969
      Modulation of p75(NTR) prevents diabetes- and proNGF-induced retinal inflammation and blood-retina barrier breakdown in mice and rats.
      Mysona, BA; Al-Gayyar, MM; Matragoon, S; Abdelsaid, MA; El-Azab, MF; Saragovi, HU; El-Remessy, AB
      Diabetologia  56  2329-39  2013

      Show Abstract
      23918145 23918145
      Recurrent long-lasting tethering reduces BDNF protein levels in the dorsal hippocampus and frontal cortex in pigs.
      J De Vry,J Prickaerts,M Jetten,M Hulst,H W M Steinbusch,D L A van den Hove,T Schuurman,F J van der Staay
      Hormones and behavior  62  2012

      Show Abstract
      22584108 22584108

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