06-820 | Anti-phospho-Adducin (Ser662) Antibody

06-820
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-820
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Adducin (Ser662) Antibody
      References
      Product Information
      FormatAffinity Purified
      Presentation0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C.
      Applications
      ApplicationThis Anti-phospho-Adducin (Ser662) Antibody is validated for use in WB for the detection of phospho-Adducin (Ser662).
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide (C-KKFRTPpSFLKKNK) corresponding to amino acids 656-668 of human gamma-Adducin
      HostRabbit
      Specificityphosphorylated serine 662 of γ-Adducin, phosphorylated serine 724 of α-Adducin, phosphorylated serine 713 of β-Adducin
      IsotypeIgG
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAdducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
      Gene Symbol
      • ADD3
      • adducin-like
      • ADDL
      • Gamma-adducin
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UEY8 # Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
      SIZE: 706 amino acids; 79155 Da
      SUBUNIT: Heterodimer of an alpha and a gamma subunit.
      TISSUE SPECIFICITY: Heart only expresses isoform 1.
      DOMAIN:SwissProt: Q9UEY8 Comprised of three regions: a N-terminal protease- resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
      PTM: Sumoylated.
      SIMILARITY: Belongs to the aldolase class II family. Adducin subfamily.
      Molecular Weight80kDa, 120kDa, 110kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells or on canine MDCK cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Adducin (Ser662) (rabbit immunoaffinity purified IgG) - 2385013 2385013
      Anti-phospho-Adducin (Ser662) (rabbit immunoaffinity purified IgG) 2912589
      Anti-phospho-Adducin (Ser662) (rabbit immunoaffinity purified IgG) - 2349601 2349601
      Anti-phospho-Adducin (Ser662) - 0610043181 0610043181
      Anti-phospho-Adducin (Ser662) - 0702051814 0702051814
      Anti-phospho-Adducin (Ser662) - 17560 17560
      Anti-phospho-Adducin (Ser662) - 23970 23970
      Anti-phospho-Adducin (Ser662) -2606867 2606867

      References

      Reference overviewPub Med ID
      Transformation-sensitive changes in expression, localization, and phosphorylation of adducins in renal proximal tubule epithelial cells.
      Fowler, L, et al.
      Cell Growth Differ., 9: 177-84 (1998)  1998

      Show Abstract
      9486854 9486854
      35H, a sequence isolated as a protein kinase C binding protein, is a novel member of the adducin family.
      Dong, L, et al.
      J. Biol. Chem., 270: 25534-40 (1995)  1995

      Show Abstract
      7592723 7592723