05-669 | Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6

100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, CaELISA, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-669
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6
      Alternate Names
      • Protein kinase B
      • RAC-alpha serine/threonine-protein kinase
      • murine thymoma viral (v-akt) oncogene homolog-1
      • rac protein kinase alpha
      • v-akt murine thymoma viral oncogene homolog 1
      Background InformationThe serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.
      Product Information
      • PDGF treated NIH-3T3 cell lysates.
      PresentationPurified mouse monoclonal IgG1κ lyophilized from 1 mL 2X PBS, PEG, sucrose, and 0.09% sodium azide.
      Reconstitute with 1 mL H2O (15 min. at RT). Concentration after reconstitution: 100 µg/mL
      ApplicationThis Anti-Akt1 Antibody, clone 11E6 is validated for use in ELISA, WB for the detection of phospho-Akt1/PKBα (Ser473).
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesELISA:
      A previous lot was recommended at 0.05 mg/mL by an independent laboratory for ELISA.
      Biological Information
      ImmunogenKLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes phosphorylated Akt1/PKBα at Ser473, Mr 60 kDa. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Canine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • MGC99656
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      • AKT
      • Phosphorylation
      Purification MethodPurified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight60 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF.

      Western Blot Analysis: 0.5-2 µg/mL of this antibody detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 100 ng/mL PDGF for 20 minutes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Akt1/PKB (Ser473), clone 11E6 - DAM1598781 DAM1598781
      Anti-phospho-Akt1/PKB (Ser473), clone 11E6 - DAM1661040 DAM1661040
      Anti-phospho-Akt1/PKBa (Ser473), clone 11E6 - 1938980 1938980
      Anti-phospho-Akt1/PKBa (Ser473), clone 11E6 - 2004511 2004511
      Anti-phospho-Akt1/PKBa (Ser473), clone 11E6 - 2043184 2043184
      Anti-phospho-Akt1/PKBa (Ser473), clone 11E6 - 2089910 2089910
      Anti-phospho-Akt1/PKBa (Ser473), clone 11E6 - 2167135 2167135
      Anti-phospho-Akt1/PKBa (Ser473), clone 11E6 - DAM1493402 DAM1493402
      Anti-phospho-Akt1/PKBa (Ser473), clone 11E6 - DAM1713174 DAM1713174
      Anti-phospho-Akt1/PKBa (Ser473), clone 11E6 - DAM17347596 DAM17347596


      Reference overviewApplicationPub Med ID
      AMPA Receptor-mTOR Activation is Required for the Antidepressant-Like Effects of Sarcosine during the Forced Swim Test in Rats: Insertion of AMPA Receptor may Play a Role.
      Chen, KT; Tsai, MH; Wu, CH; Jou, MJ; Wei, IH; Huang, CC
      Frontiers in behavioral neuroscience  9  162  2015

      Show Abstract
      26150775 26150775
      Mechanisms by which low glucose enhances the cytotoxicity of metformin to cancer cells both in vitro and in vivo.
      Zhuang, Y; Chan, DK; Haugrud, AB; Miskimins, WK
      PloS one  9  e108444  2014

      Show Abstract
      25254953 25254953
      Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer.
      Jones, LW; Antonelli, J; Masko, EM; Broadwater, G; Lascola, CD; Fels, D; Dewhirst, MW; Dyck, JR; Nagendran, J; Flores, CT; Betof, AS; Nelson, ER; Pollak, M; Dash, RC; Young, ME; Freedland, SJ
      Journal of applied physiology (Bethesda, Md. : 1985)  113  263-72  2012

      Show Abstract
      Western Blotting22604887 22604887
      GLUT4 and glycogen synthase are key players in bed rest-induced insulin resistance.
      Biensø, RS; Ringholm, S; Kiilerich, K; Aachmann-Andersen, NJ; Krogh-Madsen, R; Guerra, B; Plomgaard, P; van Hall, G; Treebak, JT; Saltin, B; Lundby, C; Calbet, JA; Pilegaard, H; Wojtaszewski, JF
      Diabetes  61  1090-9  2012

      Show Abstract
      Western Blotting22403297 22403297
      Inhibition of Invasion and Migration by Newly Synthesized Quinazolinone MJ-29 in Human Oral Cancer CAL 27 Cells through Suppression of MMP-2/9 Expression and Combined Down-regulation of MAPK and AKT Signaling.
      Chi-Cheng Lu,Jai-Sing Yang,Jo-Hua Chiang,Mann-Jen Hour,Sakae Amagaya,Kung-Wen Lu,Jing-Pin Lin,Nou-Ying Tang,Tsung-Han Lee,Jing-Gung Chung
      Anticancer research  32  2012

      Show Abstract
      22753753 22753753
      Activated protein C has a protective effect against myocardial I/R injury by improvement of endothelial function and activation of AKT1.
      Maehata, Y; Miyagawa, S; Sawa, Y
      PloS one  7  e38738  2012

      Show Abstract
      22916090 22916090
      Akt1 deficiency modulates reward learning and reward prediction error in mice.
      Y-C Chen,Y-W Chen,Y-F Hsu,W-T Chang,C K Hsiao,M-Y Min,W-S Lai
      Genes, brain, and behavior  11  2012

      Show Abstract
      22151747 22151747
      P21 (waf1/cip1) is required for non-small cell lung cancer sensitive to Gefitinib treatment.
      Zhao YF, Wang CR, Wu YM, Ma SL, Ji Y, Lu YJ
      Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie  65  151-6. Epub 2011 May 5.  2011

      Show Abstract
      21616632 21616632
      Thermogenic activation induces FGF21 expression and release in brown adipose tissue.
      Hondares E, Iglesias R, Giralt A, Gonzalez FJ, Giralt M, Mampel T, Villarroya F
      J Biol Chem  2011

      Show Abstract
      21317437 21317437
      Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo.
      Pulinilkunnil, T; He, H; Kong, D; Asakura, K; Peroni, OD; Lee, A; Kahn, BB
      The Journal of biological chemistry  286  8798-809  2011

      Show Abstract
      21209093 21209093

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      Life Science Research > Antibodies and Assays > Primary Antibodies