05-843R | Anti-phospho-Bcl-2 (Ser70) Antibody, clone 69-10C-2-10C-18, rabbit monoclonal

05-843R
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, FCRbUnconjugatedMonoclonal Antibody
      Description
      Catalogue Number05-843R
      DescriptionAnti-phospho-Bcl-2 (Ser70) Antibody, clone 69-10C-2-10C-18, rabbit monoclonal
      Alternate Names
      • B-cell CLL/lymphoma 2
      • B-cell lymphoma protein 2
      Background InformationThe Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl-2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Bcl-2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders. Bcl-2 phosphorylated at Ser-70 plays a critial role in the translocation of Bax from the cytosol to the mitochondria, and this may regulate NO-induced neuronal apoptosis. The molecular mechanism of Bcl-2 phosphorylation and its relationship to Bax is largely unknown.
      References
      Product Information
      FormatUnconjugated
      Control
      • Paclitaxel treated and untreated Jurkat cell lysate
      PresentationPurified rabbit monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% Glycerol.
      Applications
      ApplicationDetect phospho-Bcl-2 (Ser70) using this Anti-phospho-Bcl-2 Antibody, clone 69-10C-2-10C-18 validated for use in WB, IP, FC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Flow Cytometry
      Application NotesImmunoprecipitation Analysis: 1ug from a previous lot immunoprecipitated phosphorylated Bcl-2 from 100 ug of jurkat lysate treated with paciltaxel. Arrow indicates phospho-Bcl-2 (~26kD).

      Flow Cytometry Analysis: 0.1ug/ml from a previous lot was used to detect Bcl-2 in Jurkat cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phosphorylated Bcl-2 at Ser70.
      EpitopeSer70
      Clone69-10C-2-10C-18
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes phosphorylated Bcl-2 at Ser70.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]
      Gene Symbol
      • BCL2
      • Bcl-2
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

      SUBUNIT STRUCTURE: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity By similarity. Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.

      SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

      TISSUE SPECIFICTY: Expressed in a variety of tissues.

      DOMAIN: The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.

      PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) By similarity.

      Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. INVOLVEMENT IN DISEASE: A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.

      SEQUENCE SIMLARITIES: Belongs to the Bcl-2 family.
      Molecular Weight26 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in paclitaxel treated and untreated Jurkat cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected Bcl-2 on 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Bcl-2 (Ser70), clone 69- 10C-2-10C-18 - 2315621 2315621
      Anti-phospho-Bcl-2 (Ser70), clone 69- 10C-2-10C-18 - 2343838 2343838
      Anti-phospho-Bcl-2 (Ser70), clone 69- 10C-2-10C-18 - NRG1658538 NRG1658538
      Anti-phospho-Bcl-2 (Ser70), clone 69-10C-2-10C-18 - 2395687 2395687
      Anti-phospho-Bcl-2 (Ser70), clone 69-10C-2-10C-18 - 2491134 2491134
      Anti-phospho-Bcl-2 (Ser70), clone 69-10C-2-10C-18 -2608142 2608142
      Anti-phospho-Bcl-2 (Ser70), clone 69-10C-2-10C-18 -2685466 2685466
      Anti-phospho-Bcl-2 (Ser70), clone 69-10C-2-10C-18 -2689682 2689682
      Anti-phospho-Bcl-2 (Ser70), clone 69-10C-2-10C-18 -2890800 2890800
      Anti-phospho-Bcl-2 (Ser70), clone 69-10C-2-10C-18 Monoclonal Antibody 2922292

      References

      Reference overviewPub Med ID
      Aβ1-42 monomers or oligomers have different effects on autophagy and apoptosis.
      Guglielmotto, M; Monteleone, D; Piras, A; Valsecchi, V; Tropiano, M; Ariano, S; Fornaro, M; Vercelli, A; Puyal, J; Arancio, O; Tabaton, M; Tamagno, E
      Autophagy  10  1827-43  2014

      Show Abstract
      25136804 25136804
      Contribution of Bcl-2 phosphorylation to Bak binding and drug resistance.
      Dai, Haiming, et al.
      Cancer Res., 73: 6998-7008 (2013)  2013

      Show Abstract
      24097825 24097825

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies