04-792 | Anti-phospho-CENP-A (Ser7) Antibody, clone NL41, rabbit monoclonal

04-792
100 µL  
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      Overview

      Replacement Information
      Replacement InformationCat. # 05-792. 05-792 will only be availabe on a custom basis.

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HMplex, WB, ICCRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-792
      Replaces05-792
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-CENP-A (Ser7) Antibody, clone NL41, rabbit monoclonal
      OverviewCENP-A is a variant version of histone H3 found only at centromeres. It is phosphorylated at serine 7 during mitosis.
      Background InformationCentromere protein A (CENP-A) is a 17 kDa centromere-specific histone variant with 62% amino acids homology to the C-terminal of histone H3. Localized in the centromere, it plays a central role in the centromere-specific chromatin formation. The depletion of histone H3 at the CENP-A binding domain suggests CENP-A to be a possible replacement for histone H3 in the packaging process of α-satellite DNA into primary chromation structure. CENP-A is essential in the formation of specialized nucleosomes at the centromere, implicating CENP-A as a centromere-specific epigenetic marker.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Acid extracted proteins from colcemid-arrested HeLa cells
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationAnti-phospho-CENP-A (Ser7) Antibody, clone NL41 is a high quality Rabbit Monoclonal Antibody for the detection of phospho-CENP-A (Ser7) & has been validated in Mplex, WB, ICC.
      Key Applications
      • Multiplexing
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      Immunogenpeptide containing the sequence RRpSRK in which pS is phospho-serine corresponding to amino acid 7 of human CENP-A (centromere protein A)
      Cloneclone NL41
      HostRabbit
      SpecificityCENP-A phosphorylated on serine 7
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Others not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCentromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
      Gene Symbol
      • CENPA
      • CENP-A
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P49450 # Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
      SIZE: 140 amino acids; 15991 Da
      SUBUNIT: Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with herpes virus HSV-1 ICP0 protein.
      SUBCELLULAR LOCATION: Nucleus. Centromere. Kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres.
      DOMAIN: SwissProt: P49450 The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containinf CENPA and is necessary and sufficient to mediate the localization into centromeres.
      PTM: Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway. & Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.DISEASE:SwissProt: P49450 # Antibodies against CENPA are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins.
      SIMILARITY: Belongs to the histone H3 family.
      Molecular Weight~17kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid extracted proteins from colcemid-arrested HeLa cells (Catalog #17-306)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-CENP-A (Ser7), -2788177 2788177
      Anti-phospho-CENP-A (Ser7), clone NL41 2477905
      Anti-phospho-CENP-A (Ser7), clone NL41 (rabbit monoclonal) Monoclonal Antibody Q2922342
      Anti-phospho-CENP-A (Ser7), clone NL41 - DAM1498962 DAM1498962
      Anti-phospho-CENP-A (Ser7), clone NL41 -2832235 2832235

      References

      Reference overviewApplicationSpeciesPub Med ID
      A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B.
      De Antoni, A; Maffini, S; Knapp, S; Musacchio, A; Santaguida, S
      The Journal of cell biology  199  269-84  2012

      Show Abstract
      Immunofluorescence23071153 23071153
      Cdc48/p97-Ufd1-Npl4 antagonizes Aurora B during chromosome segregation in HeLa cells.
      Dobrynin, G; Popp, O; Romer, T; Bremer, S; Schmitz, MH; Gerlich, DW; Meyer, H
      Journal of cell science  124  1571-80  2011

      Show Abstract
      21486945 21486945
      Human RASSF7 regulates the microtubule cytoskeleton and is required for spindle formation, Aurora B activation and chromosomal congression during mitosis.
      Recino, A; Sherwood, V; Flaxman, A; Cooper, WN; Latif, F; Ward, A; Chalmers, AD
      The Biochemical journal  430  207-13  2010

      Show Abstract Full Text Article
      ImmunofluorescenceHuman20629633 20629633

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies