06-519 Anti-phospho-CREB (Ser133) Antibody

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, HtEMSA, IHC, IH(P), IP, WBRbPurifiedPolyclonal Antibody
      Catalogue Number06-519
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-CREB (Ser133) Antibody
      Alternate Names
      • active transcription factor CREB
      • cAMP responsive element binding protein 1
      • cAMP-response element-binding protein-1
      • transactivator protein
      Background InformationCREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.
      Product Information
      • Forskolin-stimulated WI-38 nuclear extracts.
      PresentationPurified rabbit IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4) 15 mM NaCl, and 0.05% sodium azide with 30% glycerol.
      ApplicationUse Anti-phospho-CREB (Ser133) Antibody (rabbit polyclonal antibody) is highly published and validated in EMSA, IHC,IHC(P), IP, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      10 μL of a previous lot immunoprecipitated phosphorylated CREB from 500 μg of cells treated with 50 μM forskolin.

      A 1:1000 dilution of previous lots detected phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.

      Gel Shift (EMSA):
      This antibody has been shown to gel shift by independent labs.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes p43 phosphorylated CREB. May also recognize p30 and p38 kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Hamster
      Species Reactivity NoteReacts with human, hamster, mouse and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
      Gene Symbol
      • CREB1
      • CREB
      • MGC9284
      • Phosphorylation
      Purification MethodPurified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column.
      UniProt Number
      UniProt SummaryFUNCTION:This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
      SUBUNIT: Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133, binds CREBBP By similarity.
      SUBCELLULAR: Nucleus.
      PMT:Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: Belongs to the bZIP family.
      Contains 1 bZIP domain.
      Contains 1 KID (kinase-inducible) domain
      Molecular WeightDetects CREB only when phosphorylated on Ser133 (~43 kDa). May recognize ATF-1 (~38 kDa) and CREM (~30 kDa) as the two proteins share significant homology to the phosphorylated immunizing peptide.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on lysates from mouse NIH/3T3 cells treated with 50 mM forskolin.

      Western Blot Analysis: This antibody detected CREB when phosphorylated on Ser133 at 1:1,000 dilution in forskolin treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
      Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho CREB (Ser133) - 24912142491214
      Anti-phospho CREB (Ser133) - 30187733018773
      Anti-phospho CREB (Ser133) -25541072554107
      Anti-phospho CREB (Ser133) -25817492581749
      Anti-phospho CREB (Ser133) -26104172610417
      Anti-phospho CREB (Ser133) -26203022620302
      Anti-phospho CREB (Ser133) -26991952699195
      Anti-phospho CREB (Ser133) -27217152721715
      Anti-phospho CREB (Ser133) -27571602757160
      Anti-phospho CREB (Ser133) -27622422762242


      Reference overviewApplicationSpeciesPub Med ID
      Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease.
      D'Souza, Y; Elharram, A; Soon-Shiong, R; Andrew, RD; Bennett, BM
      Molecular brain  8  27  2015

      Show Abstract
      25910195 25910195
      Region and context-specific intracellular responses associated with cocaine-induced conditioned place preference expression.
      Nygard, SK; Klambatsen, A; Balouch, B; Quinones-Jenab, V; Jenab, S
      Neuroscience  287  1-8  2015

      Show Abstract
      25522720 25522720
      Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area.
      Koo, JW; Mazei-Robison, MS; LaPlant, Q; Egervari, G; Braunscheidel, KM; Adank, DN; Ferguson, D; Feng, J; Sun, H; Scobie, KN; Damez-Werno, DM; Ribeiro, E; Peña, CJ; Walker, D; Bagot, RC; Cahill, ME; Anderson, SA; Labonté, B; Hodes, GE; Browne, H; Chadwick, B; Robison, AJ; Vialou, VF; Dias, C; Lorsch, Z; Mouzon, E; Lobo, MK; Dietz, DM; Russo, SJ; Neve, RL; Hurd, YL; Nestler, EJ
      Nature neuroscience  18  415-22  2015

      Show Abstract
      25643298 25643298
      Young blood reverses age-related impairments in cognitive function and synaptic plasticity in mice.
      Villeda, SA; Plambeck, KE; Middeldorp, J; Castellano, JM; Mosher, KI; Luo, J; Smith, LK; Bieri, G; Lin, K; Berdnik, D; Wabl, R; Udeochu, J; Wheatley, EG; Zou, B; Simmons, DA; Xie, XS; Longo, FM; Wyss-Coray, T
      Nature medicine  20  659-63  2014

      Show Abstract
      ImmunohistochemistryMouse24793238 24793238
      Inhibition of phosphodiesterase10A attenuates morphine-induced conditioned place preference.
      Mu, Y; Ren, Z; Jia, J; Gao, B; Zheng, L; Wang, G; Friedman, E; Zhen, X
      Molecular brain  7  70  2014

      Show Abstract
      Immunofluorescence25252626 25252626
      A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis.
      Megill, A; Lee, T; DiBattista, AM; Song, JM; Spitzer, MH; Rubinshtein, M; Habib, LK; Capule, CC; Mayer, M; Turner, RS; Kirkwood, A; Yang, J; Pak, DT; Lee, HK; Hoe, HS
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  9306-18  2013

      Show Abstract
      23719799 23719799
      M1 muscarinic receptor activation mediates cell death in M1-HEK293 cells.
      Graham, ES; Woo, KK; Aalderink, M; Fry, S; Greenwood, JM; Glass, M; Dragunow, M
      PloS one  8  e72011  2013

      Show Abstract
      24023725 24023725
      AAD-2004 Attenuates Progressive Neuronal Loss in the Brain of Tg-betaCTF99/B6 Mouse Model of Alzheimer Disease.
      Baek, IS; Kim, TK; Seo, JS; Lee, KW; Lee, YA; Cho, J; Gwag, BJ; Han, PL
      Experimental neurobiology  22  31-7  2013

      Show Abstract
      23585720 23585720
      Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification.
      Regard, JB; Malhotra, D; Gvozdenovic-Jeremic, J; Josey, M; Chen, M; Weinstein, LS; Lu, J; Shore, EM; Kaplan, FS; Yang, Y
      Nature medicine  19  1505-12  2013

      Show Abstract
      Western Blotting24076664 24076664
      Sexually dimorphic intracellular responses after cocaine-induced conditioned place preference expression.
      Nygard, SK; Klambatsen, A; Hazim, R; Eltareb, MH; Blank, JC; Chang, AJ; Quinones-Jenab, V; Jenab, S
      Brain research  1520  121-33  2013

      Show Abstract
      23665060 23665060


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