05-533 | Anti-phospho-CaM Kinase II Antibody, α subunit, (Thr286), clone 22B1

05-533
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-533
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-CaM Kinase II Antibody, α subunit, (Thr286), clone 22B1
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect phospho-CaM Kinase II using this Anti-phospho-CaM Kinase II Antibody, α subunit, (Thr286), clone 22B1 validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to residuues 281-294 of rat Cam Kinase II alpha-subunit; recognizes phosphorylated CaM Kinase II on Threonine 286
      Clone22B1
      HostMouse
      Specificityphospho-CaM Kinase II α subunit
      IsotypeIgG1
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the Serine/Threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This enzyme is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene.
      Gene Symbol
      • CAMK2A
      • KIAA0968
      • CAMKA
      Modifications
      • Phosphorylation
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UQM7 # CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity).
      SIZE: 478 amino acids; 54030 Da
      SUBUNIT: CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1 and SYNGAP1 (By similarity).
      SUBCELLULAR LOCATION: Cell junction, synapse, presynaptic cell membrane (By similarity). Cell junction, synapse (By similarity). Note=Postsynaptic lipid rafts (By similarity).
      SIMILARITY: SwissProt: Q9UQM7 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. & Contains 1 protein kinase domain.
      Molecular Weight50kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on rat brain microsomal protein preparation
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-CaM Kinase II (Thr286) (mouse monoclonal IgG1) - 2446890 2446890
      Anti-phospho-CaM Kinase II (Thr286) (mouse monoclonal IgG1) 2983270
      Anti-phospho-CaM Kinase II (Thr286) (mouse monoclonal IgG1) 2890793
      Anti-phospho-CaM Kinase II (Thr286) (mouse monoclonal IgG1) - 2168324 2168324
      Anti-phospho-CaM Kinase II (Thr286) (mouse monoclonal IgG1) - 2297220 2297220
      Anti-phospho-CaM Kinase II (Thr286) - 2032284 2032284
      Anti-phospho-CaM Kinase II (Thr286) - 2510365 2510365
      Anti-phospho-CaM Kinase II (Thr286) - DAM1713170 DAM1713170
      Anti-phospho-CaM Kinase II (Thr286) -2603820 2603820
      Anti-phospho-CaM Kinase II (Thr286) -2683849 2683849

      References

      Reference overviewPub Med ID
      Bone marrow stroma-secreted cytokines protect JAK2(V617F)-mutated cells from the effects of a JAK2 inhibitor.
      Manshouri, T; Estrov, Z; Quintás-Cardama, A; Burger, J; Zhang, Y; Livun, A; Knez, L; Harris, D; Creighton, CJ; Kantarjian, HM; Verstovsek, S
      Cancer research  71  3831-40  2011

      Show Abstract
      21512135 21512135
      Comparative biology of cystic fibrosis animal models.
      Fisher, JT; Zhang, Y; Engelhardt, JF
      Methods in molecular biology (Clifton, N.J.)  742  311-34  2011

      Show Abstract
      21547741 21547741
      Maternal hypothyroxinemia impairs spatial learning and synaptic nature and function in the offspring.
      Opazo, MC; Gianini, A; Pancetti, F; Azkcona, G; Alarcón, L; Lizana, R; Noches, V; Gonzalez, PA; Marassi, MP; Porto, M; Mora, S; Rosenthal, D; Eugenin, E; Naranjo, D; Bueno, SM; Kalergis, AM; Riedel, CA
      Endocrinology  149  5097-106  2008

      Show Abstract
      18566112 18566112
      Overexpression of calbindin-D28K in hippocampal progenitor cells increases neuronal differentiation and neurite outgrowth.
      Kim, JH; Lee, JA; Song, YM; Park, CH; Hwang, SJ; Kim, YS; Kaang, BK; Son, H
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  20  109-11  2006

      Show Abstract
      16278289 16278289
      Integrins modulate fast excitatory transmission at hippocampal synapses.
      Kramár, EA; Bernard, JA; Gall, CM; Lynch, G
      The Journal of biological chemistry  278  10722-30  2003

      Show Abstract
      12524441 12524441
      Autophosphorylation of type II CaM kinase in hippocampal neurons: localization of phospho- and dephosphokinase with complementary phosphorylation site-specific antibodies.
      Patton, B L, et al.
      Mol. Biol. Cell, 4: 159-72 (1993)  1993

      Show Abstract
      8443414 8443414

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies