04-282 | Anti-phospho-EGFR (Thr654) Antibody, clone 3F2

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, RELISA, IP, WBMPurifiedMonoclonal Antibody
      Catalogue Number04-282
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Thr654) Antibody, clone 3F2
      Alternate Names
      • ERBB
      • ERBB1
      Product Information
      • Includes PMA and pervanadate treated Hep2G cell lysate as a positive control
      Presentation100 µg (0.1 mg/ml) of mouse monoclonal IgG1 in PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol
      ApplicationDetect phospho-EGFR (Thr654) using this Anti-phospho-EGFR (Thr654) Antibody, clone 3F2 validated for use in ELISA, IP & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 654 in human EGFR
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes phosphorylated EGFR on Threonine 654
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      • Phosphorylation
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-phospho-EGFR (Thr654), clone 3F2 2913492
      Anti-phospho-EGFR (Thr654), clone 3F2 (Mouse monoclonal IgG1) - 0138S0102 0138S0102
      Anti-phospho-EGFR (Thr654), clone 3F2 - 2433299 2433299
      Anti-phospho-EGFR (Thr654), clone 3F2 - 0138S0102 0138S0102
      Anti-phospho-EGFR (Thr654), clone 3F2 - 2070809 2070809
      Anti-phospho-EGFR (Thr654), clone 3F2 - 2191916 2191916
      Anti-phospho-EGFR (Thr654), clone 3F2 - 2949486 2949486
      Anti-phospho-EGFR (Thr654), clone 3F2 - DAM1564596 DAM1564596
      Anti-phospho-EGFR (Thr654), clone 3F2 - DAM1731537 DAM1731537
      Anti-phospho-EGFR (Thr654), clone 3F2 - DAM1774736 DAM1774736


      Reference overviewPub Med ID
      Phosphorylation of Thr654 but not Thr669 within the juxtamembrane domain of the EGF receptor inhibits calmodulin binding
      Aifa, Sami, et al
      Biochem Biophys Res Commun, 347:381-7 (2006)  2006

      16793002 16793002
      Platelet-derived growth factor mimics phorbol diester action on epidermal growth factor receptor phosphorylation at threonine-654.
      Davis, R J and Czech, M P
      Proc. Natl. Acad. Sci. U.S.A., 82: 4080-4 (1985)  1985

      Show Abstract
      2987962 2987962
      Protein kinase C phosphorylation of the EGF receptor at a threonine residue close to the cytoplasmic face of the plasma membrane
      Hunter, T, et al
      Nature, 311:480-3 ()  1984

      6090944 6090944

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      Life Science Research > Antibodies and Assays > Primary Antibodies