04-283 | Anti-phospho-EGFR (Tyr845) Antibody, clone 12A3

04-283
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MELISA, ICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-283
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Tyr845) Antibody, clone 12A3
      Alternate Names
      • ERBB
      • ERBB1
      References
      Product Information
      FormatPurified
      Control
      • Includes EGF treated Hep2G cell lysate as a positive control
      Presentation100 µg of mouse monoclonal IgG1 lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose
      Applications
      ApplicationDetect phospho-EGFR (Tyr845) using this Anti-phospho-EGFR (Tyr845) Antibody, clone 12A3 validated for use in ELISA, IC, IP & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyr 845 in human EGFR.
      Clone12A3
      HostMouse
      SpecificityRecognizes phosphorylated EGFR on Tyrosine 845.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Modifications
      • Phosphorylation
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-EGFR (Tyr845), -2762363 2762363
      Anti-phospho-EGFR (Tyr845), clone 12A3 - 0116S0108 0116S0108
      Anti-phospho-EGFR (Tyr845), clone 12A3 - 1983658 1983658
      Anti-phospho-EGFR (Tyr845), clone 12A3 - 2224373 2224373
      Anti-phospho-EGFR (Tyr845), clone 12A3 - 2292289 2292289
      Anti-phospho-EGFR (Tyr845), clone 12A3 - 2377665 2377665
      Anti-phospho-EGFR (Tyr845), clone 12A3 - DAM1487584 DAM1487584
      Anti-phospho-EGFR (Tyr845), clone 12A3 - DAM1519012 DAM1519012
      Anti-phospho-EGFR (Tyr845), clone 12A3 - DAM1606779 DAM1606779
      Anti-phospho-EGFR (Tyr845), clone 12A3 - NG1846082 NG1846082

      References

      Reference overviewPub Med ID
      Phosphorylation of Y845 on the epidermal growth factor receptor mediates binding to the mitochondrial protein cytochrome c oxidase subunit II
      Boerner, Julie L, et al
      Mol Cell Biol, 24:7059-71 (2004)  2004

      15282306 15282306
      Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines
      Moro, Laura, et al
      J Biol Chem, 277:9405-14 (2002)  2002

      11756413 11756413
      c-Src-mediated phosphorylation of the epidermal growth factor receptor on Tyr845 and Tyr1101 is associated with modulation of receptor function
      Biscardi, J S, et al
      J Biol Chem, 274:8335-43 (1999)  1999

      10075741 10075741

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies