05-797R | Anti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonal

05-797R
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RMplex, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-797R
      Replaces05-797
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonal
      Alternate Names
      • Erk1
      • Erk2
      • p44-MAPK
      • p42-MAPK
      • MAPK2
      • PRKM2
      • p41mapk
      • PRKM1
      • p44
      • ERK2
      • P42MAPK
      • ERK
      • p42
      • MAPK1
      • ERT1
      • ERT2
      • Mitogen-activated protein kinase
      • MAP Kinase 2
      • MAP Kianse 1
      • MAPK
      Background InformationErk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).
      References
      Product Information
      FormatPurified
      Control
      • PDGF-treated NIH/3T3 cell lysates
      PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-phospho-Erk1/2 (Thr202/Tyr204 Antibody, Thr185/Tyr187), recombinant clone AW39R detects level of phospho-Erk1/2 (Thr202/Tyr204 & has been published & validated for use in Mplex & WB.
      Key Applications
      • Multiplexing
      • Western Blotting
      Biological Information
      ImmunogenPhosphorylated peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated
      EpitopepTEpY in the activation loop
      CloneAW39R
      HostRabbit
      SpecificityRecognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • Erk1
      • Erk2
      • p44-MAPK
      • p42-MAPK
      • MAPK2
      • PRKM2
      • p41mapk
      • PRKM1
      • p44
      • ERK2
      • P42MAPK
      • ERK
      • p42
      • MAPK1
      • ERT1
      • ERT2
      • Mitogen-activated protein kinase
      • MAP Kinase 2
      • MAP Kianse 1
      • MAPK
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 360 amino acids; 41390 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular WeightErk1 (p44) (~44 kDa) and Erk2 (p42) (~42 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on lysate from PDGF-treated NIH/3T3 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Erk 1/2 2470807
      Anti-phospho-Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - 2137038 2137038
      Anti-phospho-Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - 2382623 2382623
      Anti-phospho-Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - 2452191 2452191
      Anti-phospho-Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - 1987096 1987096
      Anti-phospho-Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - 2049817 2049817
      Anti-phospho-Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - 2107273 2107273
      Anti-phospho-Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - 2161252 2161252
      Anti-phospho-Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - 2225197 2225197
      Anti-phospho-Erk 1/2 (Thr202/Tyr204, Thr185/Tyr187), recombinant clone AW39R - 2291700 2291700

      References

      Reference overviewPub Med ID
      AMPA Receptor-mTOR Activation is Required for the Antidepressant-Like Effects of Sarcosine during the Forced Swim Test in Rats: Insertion of AMPA Receptor may Play a Role.
      Chen, KT; Tsai, MH; Wu, CH; Jou, MJ; Wei, IH; Huang, CC
      Frontiers in behavioral neuroscience  9  162  2015

      Show Abstract
      26150775 26150775
      Pdx-1 activates islet α- and β-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2.
      Hayes, HL; Moss, LG; Schisler, JC; Haldeman, JM; Zhang, Z; Rosenberg, PB; Newgard, CB; Hohmeier, HE
      Molecular and cellular biology  33  4017-29  2013

      Show Abstract
      23938296 23938296
      N-acetylcysteine protects against apoptosis through modulation of group I metabotropic glutamate receptor activity.
      Sun, L; Gu, L; Wang, S; Yuan, J; Yang, H; Zhu, J; Zhang, H
      PloS one  7  e32503  2012

      Show Abstract
      22442667 22442667

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      Life Science Research > Antibodies and Assays > Primary Antibodies