05-793 | Anti-phospho-Estrogen Receptor alpha (Ser118) Antibody, clone NL44, rabbit monoclonal

05-793
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-793
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Estrogen Receptor alpha (Ser118) Antibody, clone NL44, rabbit monoclonal
      References
      Product Information
      FormatPurified
      Presentation0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 100mM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%
      Applications
      ApplicationAnti-phospho-Estrogen Receptor alpha (Ser118) Antibody, clone NL44 is a Rabbit Monoclonal Antibody for detection of ER alpha also known as Estrogen receptor alpha & has been tested in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide containing the sequence QLpSPF in which pS corresponds to phosphoserine at residue 118 of human estrogen receptor alpha
      CloneNL44
      HostRabbit
      Specificityphospho-estrogen receptor a (Ser118)
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NotePredicted to cross-react with camel, mouse, pig and Syrian hamster based on sequence homology
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
      Gene Symbol
      • ESR1
      • Era
      • ER-alpha
      • ESR
      • ER
      • NR3A1
      • ESRA
      • DKFZp686N23123
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
      SIZE: 595 amino acids; 66216 Da
      SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
      PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
      MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.
      Molecular Weight~66kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from MCF-7 cells treated with estradiol and EGF, and recombinant estrogen receptor alpha phosphorylated in vitro with MAP Kinase 2/Erk2, active (Catalog #14-173)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Estrogen Receptor #945; (Ser118), clone NL44 - 26858 26858
      Anti-phospho-Estrogen Receptor α (Ser118), clone NL44 (rabbit monoclonal IgG) 2988811
      Anti-phospho-Estrogen Receptor α (Ser118), clone NL44 -2592099 2592099
      Anti-phospho-Estrogen Receptor α (Ser118), clone NL44 -2601672 2601672
      Anti-phospho-Estrogen Receptor α (Ser118), clone NL44 -2757884 2757884
      Anti-phospho-Estrogen Receptor α (Ser118), clone NL44 -2774112 2774112
      AntiAnti -phospho -Estrogen Receptor Estrogen Receptor -2786340 2786340

      References

      Reference overviewPub Med ID
      Phosphorylation of human estrogen receptor alpha at serine 118 by two distinct signal transduction pathways revealed by phosphorylation-specific antisera.
      Chen, Dongsheng, et al.
      Oncogene, 21: 4921-31 (2002)  2002

      Show Abstract
      12118371 12118371
      Estradiol-induced phosphorylation of serine 118 in the estrogen receptor is independent of p42/p44 mitogen-activated protein kinase.
      Joel, P B, et al.
      J. Biol. Chem., 273: 13317-23 (1998)  1998

      Show Abstract
      9582378 9582378

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies