05-643 | Anti-phospho-GSK3β (Ser9) Antibody, clone 2D3

05-643
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, REMSA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-643
      Replaces04-1075
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-GSK3β (Ser9) Antibody, clone 2D3
      References
      Product Information
      FormatPurified
      Presentation100µg of thiophilic and size exclusion chromatography purified mouse IgG1κ in 100µL of antibody storage buffer (PBS with PEG, Sucrose, and 0.09% sodium azide) and 50% glycerol.
      Applications
      ApplicationAnti-phospho-GSK3β (Ser9) Antibody, clone 2D3 is an antibody against phospho-GSK3β (Ser9) for use in EMSA & WB.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to amino acids 6-12 (RTT[pS]AE) of human GSK3beta
      Clone2D3
      HostMouse
      SpecificityGSK3beta
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]
      Gene Symbol
      • GSK3B
      Modifications
      • Phosphorylation
      Purification MethodThiophilic adsorption chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P49841 # Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
      SIZE: 420 amino acids; 46744 Da
      SUBUNIT: Monomer (By similarity). Interacts with NIN and CABYR.
      TISSUE SPECIFICITY: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
      PTM: Phosphorylated by AKT1 and ILK1.
      SIMILARITY: SwissProt: P49841 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain.
      Molecular Weight46kDa (GSK3β) and 51kDa (GSK3α)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on L6 cells stimulated with IGF-1
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-GSK3B (Ser9), clone 2D3 (mouse monoclonal IgG1K) 2891402
      Anti-phospho-GSK3B (Ser9), clone 2D3 (mouse monoclonal IgG1k) 2938844
      Anti-phospho-GSK3b (Ser9), clone 2D3 (mouse monoclonal IgG1k) - 00980503 00980503
      Anti-phospho-GSK3b (Ser9), clone 2D3 (mouse monoclonal IgG1k) - 0098S0502 0098S0502
      Anti-phospho-GSK3b (Ser9), clone 2D3 (mouse monoclonal IgG1k) - 1963623 1963623
      Anti-phospho-GSK3b (Ser9), clone 2D3 (mouse monoclonal IgG1k) - 2023281 2023281
      Anti-phospho-GSK3b (Ser9), clone 2D3 - DAM1636072 DAM1636072
      Anti-phospho-GSK3beta; (Ser9), clone 2D3 - 22152 22152
      Anti-phospho-GSK3beta; (Ser9), clone 2D3 - 25187 25187
      Anti-phospho-GSK3beta; (Ser9), clone 2D3 - 27291 27291

      References

      Reference overviewApplicationPub Med ID
      Inhibition of cyclin-dependent kinase 5 but not of glycogen synthase kinase 3-β prevents neurite retraction and tau hyperphosphorylation caused by secretable products of human T-cell leukemia virus type I-infected lymphocytes.
      Maldonado, H; Ramírez, E; Utreras, E; Pando, ME; Kettlun, AM; Chiong, M; Kulkarni, AB; Collados, L; Puente, J; Cartier, L; Valenzuela, MA
      Journal of neuroscience research  89  1489-98  2011

      Show Abstract
      21671254 21671254
      Measuring GSK3 expression and activity in cells.
      Adam R Cole,Calum Sutherland
      Methods in molecular biology (Clifton, N.J.)  468  2008

      Show Abstract
      19099245 19099245
      Inhibition of SIRT1 reactivates silenced cancer genes without loss of promoter DNA hypermethylation.
      Pruitt, K; Zinn, RL; Ohm, JE; McGarvey, KM; Kang, SH; Watkins, DN; Herman, JG; Baylin, SB
      PLoS genetics  2  e40  2006

      Show Abstract
      Western Blotting16596166 16596166