04-823 | Anti-phospho-GluR1 (Ser831) Antibody, clone N453, rabbit monoclonal

04-823
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M, RWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-823
      Replaces05-823
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-GluR1 (Ser831) Antibody, clone N453, rabbit monoclonal
      Alternate Names
      • AMPA 1
      • AMPA-selective glutamate receptor 1
      • Glutamate receptor ionotropic
      • AMPA 1
      • glutamate receptor, ionotropic
      • AMPA 1
      Background InformationL glutamate is the major excitatory neurotransmitter in the central nervous system. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors that have been divided into 3 groups. Group I includes GRM1 and these receptors have been shown to activate phospholipase C. GRM1 expression has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Rat brain
      PresentationCultured supernantant in 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-GluR1 (Ser831) Antibody, clone N453 detects level of phospho-GluR1 (Ser831) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis:
      A 1:1,000-1:4,000 dilution of this lot detected phosphorylated GluR1 from a rat brain microsomal preparation (Catalog # 12-144).
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...QQ[pS]IN… in which pS corresponds to phospho-serine at position 831 of the GluR1 mature chain. Sequence is identical in human, rat, mouse and chicken.
      CloneN453
      HostRabbit
      SpecificityRecognizes GluR1 phosphorylated on Serine 831.
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Rat
      Species Reactivity NotePredicted cross-reactivity with human, mouse and chicken
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • GRIA1
      • GluR-A
      • GluR-1
      • GLUR1
      • GluR-K1
      • GLURA
      • HBGR1
      • GLUH1
      • MGC133252
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42261 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
      SIZE: 906 amino acids; 101506 Da
      SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2 and RASGRF2 (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Interaction with CACNG2 promotes cell surface expression (By similarity).
      TISSUE SPECIFICITY: Widely expressed in brain.
      PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
      SIMILARITY: SwissProt: P42261 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
      MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
      Molecular Weight106 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by western blot
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-GluR1 (Ser831), clone N453 - 2387820 2387820
      Anti-phospho-GluR1 (Ser831), clone N453 - 2150756 2150756
      Anti-phospho-GluR1 (Ser831), -2548703 2548703
      Anti-phospho-GluR1 (Ser831), -2551205 2551205
      Anti-phospho-GluR1 (Ser831), -2575747 2575747
      Anti-phospho-GluR1 (Ser831), -2625342 2625342
      Anti-phospho-GluR1 (Ser831), -2691193 2691193
      Anti-phospho-GluR1 (Ser831), -2700780 2700780
      Anti-phospho-GluR1 (Ser831), -2726818 2726818
      Anti-phospho-GluR1 (Ser831), -2802591 2802591

      References

      Reference overviewPub Med ID
      Inhibition of adenylyl cyclase type 5 prevents L-DOPA-induced dyskinesia in an animal model of Parkinson's disease.
      Park, HY; Kang, YM; Kang, Y; Park, TS; Ryu, YK; Hwang, JH; Kim, YH; Chung, BH; Nam, KH; Kim, MR; Lee, CH; Han, PL; Kim, KS
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  11744-53  2014

      Show Abstract
      25164669 25164669
      Reactive oxygen species (ROS) modulate AMPA receptor phosphorylation and cell-surface localization in concert with pain-related behavior.
      Lee, DZ; Chung, JM; Chung, K; Kang, MG
      Pain  153  1905-15  2012

      Show Abstract
      22770842 22770842
      Identification of the Ca2+/calmodulin-dependent protein kinase II regulatory phosphorylation site in the alpha-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate-type glutamate receptor.
      Barria, A, et al.
      J. Biol. Chem., 272: 32727-30 (1997)  1997

      Show Abstract
      9407043 9407043
      Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit.
      Roche, K W, et al.
      Neuron, 16: 1179-88 (1996)  1996

      Show Abstract
      8663994 8663994
      The striatal mosaic in primates: striosomes and matrix are differentially enriched in ionotropic glutamate receptor subunits.
      Martin, L J, et al.
      J. Neurosci., 13: 782-92 (1993)  1993

      7678861 7678861
      The distribution of glutamate receptors in cultured rat hippocampal neurons: postsynaptic clustering of AMPA-selective subunits.
      Craig, A M, et al.
      Neuron, 10: 1055-68 (1993)  1993

      Show Abstract
      7686378 7686378
      Cellular localizations of AMPA glutamate receptors within the basal forebrain magnocellular complex of rat and monkey.
      Martin, L J, et al.
      J. Neurosci., 13: 2249-63 (1993)  1993

      Show Abstract
      8386757 8386757

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies