07-1520 | Anti-phospho-Grb10 (Ser501/Ser503) Antibody

07-1520
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Po, Rhesus Macaque, Eq, PmWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1520
      DescriptionAnti-phospho-Grb10 (Ser501/Ser503) Antibody
      Alternate Names
      • growth factor receptor-bound protein 10
      • OTTHUMP00000197988
      • OTTHUMP00000197989
      • OTTHUMP00000197990
      • OTTHUMP00000197991
      • OTTHUMP00000197992
      • OTTHUMP00000197993
      • OTTHUMP00000197994
      • GRB10 adapter protein
      • GRB10 adaptor protein
      • maternally expressed gene 1
      • insulin receptor-binding protein Grb-IR
      Background InformationGrowth factor receptor-bound protein 10 (Grb10) belongs to a small family of structurally related multi-domain adaptor proteins, which are known to be involved with a diverse array of functions, including cellular growth, metabolism, apoptosis, and cell migration. Grb10 is widely, but not uniformly, expressed in mammalian tissues, and there are various known isoforms of Grb10. It is shown to bind to and positively or negatively mediate signals from a number of activated receptor tyrosine kinases, including the insulin (INSR) and the insulin-like growth factor (IGF1R and IGF2R) receptors, in addition to a variety of growth factor receptors and intracellular molecules, in a context-dependent manner. In one study, mTORC1-mediated Grb10 phosphorylation is demonstrated to lead to feedback inhibition of the phosphatidylinositol-3-kinase (PI3K) and the extracellular signal-regulated, mitogen-activated protein kinase (ERK-MAPK) pathways in HEK 293T cells, in vivo. However, many aspects of the Grb10 adapter function remain unclear. Further research is still required to elucidate and validate the interactions and effects of GRB10 with various cellular targets.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Insulin untreated and treated mouse embryonic fibroblast cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-Grb10 (Ser501/Ser503) Antibody detects level of phospho-Grb10 (Ser501/Ser503) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Grb10 phosphorylated at Ser501/Ser503.
      EpitopePhosphorylated Ser501/Ser503.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Grb10 phosphorylated at Ser501/Ser503.
      Species Reactivity
      • Mouse
      • Human
      • Pig
      • Rhesus Macaque
      • Horse
      • Primate
      Species Reactivity NoteDemonstrated to react with Mouse. Predicted to react with Human, Porcine, Rhesus Macaque, Equine, and Primate based on 100% sequence homology. Other homologies: Rat (91% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified.
      Gene Symbol
      • GRB10
      • GRBIR
      • KIAA0207
      Modifications
      • Phosphorylation
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.

      SUBUNIT STRUCTURE: Interacts with ligand-activated tyrosine kinase receptors, including FGFR1, INSR, IGF1R, MET and PDGFRB in a phosphotyrosine-dependent manner through the SH2 domain By similarity. Poorly binds to the EGFR By similarity. Directly interacts with MAP3K14/NIK and is recruited to the EGFR-ERBB2 complex. Interacts with GIGYF1/PERQ1 and GIGYF2/TNRC15 By similarity. When unphosphorylated, interacts with AKT1 and when phosphorylated with YWHAE/14-3-3 epsilon. Interacts with NEDD4. Interacts with LRP6, thus interfering with the binding of AXIN1 to LRP6 By similarity. Binds to activated NRAS.

      SUBCELLULAR LOCATION: Cytoplasm.

      Molecular Weight~66 kDa observed. Uncharacterized bands detected in some lysates. UniProt describes 4 isoforms produced by alternative splicing: Isoform 1 at 62.026 kDa, Isoform 2 at 60.848 kDa, Isoform 3 at 67.231 kDa, and Isoform 4 at 66.487 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in insulin untreated and treated mouse embryonic fibroblast cell lysates.

      Western Blot Analysis: 1 µg/mL of this antibody detected Grb10 in 10 µg of insulin untreated and treated mouse embryonic fibroblast cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Grb10 (Ser501/Ser503) 2476944
      Anti-phospho-Grb10 (Ser501/Ser503) - 2360587 2360587
      Anti-phospho-Grb10 (Ser501/Ser503) - 2388976 2388976
      Anti-phospho-Grb10 (Ser501/Ser503) - 2433628 2433628
      Anti-phospho-Grb10 (Ser501/Ser503) - 1946581 1946581
      Anti-phospho-Grb10 (Ser501/Ser503) - 2026029 2026029
      Anti-phospho-Grb10 (Ser501/Ser503) - 2512132 2512132
      Anti-phospho-Grb10 (Ser501/Ser503) - NG1900682 NG1900682
      Anti-phospho-Grb10 (Ser501/Ser503) - NRG1889575 NRG1889575
      Anti-phospho-Grb10 (Ser501/Ser503) -2687782 2687782

      References

      Reference overviewPub Med ID
      Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling.
      Yu, Y; Yoon, SO; Poulogiannis, G; Yang, Q; Ma, XM; Villén, J; Kubica, N; Hoffman, GR; Cantley, LC; Gygi, SP; Blenis, J
      Science (New York, N.Y.)  332  1322-6  2011

      Show Abstract
      21659605 21659605

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies