05-636-AF555 | Anti-phospho-H2A.X Antibody (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate

05-636-AF555
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue Number05-636-AF555
      DescriptionAnti-phospho-H2A.X Antibody (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate
      Alternate Names
      • H2AXS139P
      • Histone H2A.X (phospho S139)
      Background InformationHistone H2A.X, also known as H2A/X, or H2A.X, and encoded by the gene name H2AFX and H2AX, is a variant of histone H2A and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins. As a member of the histone H2A family, histone H2A.x (H2A.x) is a variant histone H2A which replaces conventional H2A in a subset of nucleosomes. H2A.x is involved in the DNA repair of double-strand breakage (DSB) damage on nuclear DNA. After a double strand DNA break, H2A.x is rapidly phosphorylated at serine 139 by ATM kinase and becomes gamma-H2AFX. This phosphorylation step can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair (1). As a part of posttranslational modifications during apoptosis caused by severe DNA damage, high expression of phosphorylated H2A.x is considered as an accurate indicator of apoptosis.
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified mouse monoclonal IgG1 in buffer containing PBS with 15 mg/mL BSA and 0.1% azide
      Applications
      ApplicationAnti-phospho-H2A.X (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate is an antibody against phospho-H2A.X (Ser139) for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesChromatin Immunoprecipitation Analysis: A representative lot of the non-conjugated form of this antibody (Cat. # 05-636) was used to detect phospho-Histone H2A.X (Ser139) by ChIP (Meier, A. et al. (2007) EMBO J., 26:2707-18).

      Western Blot Analysis: 0.05-1 μg/mL of a representative lot of the non-conjugated form of this antibody (Cat. # 05-636) detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
      Biological Information
      ImmunogenLinear peptide corresponding to phospho-Histone H2A.X (Ser139).
      CloneJBW301
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Vertebrates based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H2AFX
      • H2AX
      • H2a/x
      • H2A/X
      • H2A.X
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells +/- Staurosporin treatment.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H2A.X (Ser139) in HeLa cells +/- Staurosporin treatment.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-H2A.X (Ser139), clone -2722597 2722597
      Anti-phospho-H2A.X (Ser139), clone -2752487 2752487
      Anti-phospho-H2A.X (Ser139), clone -2756655 2756655
      Anti-phospho-H2A.X (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate Q2483344
      Anti-phospho-H2A.X (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate 2931010
      Anti-phospho-H2A.X (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate -2626788 2626788
      Anti-phospho-H2A.X (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate -2707774 2707774
      Anti-phospho-H2A.X (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate -2893224 2893224
      Anti-phospho-H2A.X (Ser139), clone JBW30, Alexa Fluor® 555 Conjugate Monoclonal Antibody 2867923

      References

      Reference overviewPub Med ID
      Spreading of mammalian DNA-damage response factors studied by ChIP-chip at damaged telomeres.
      Meier, A; Fiegler, H; Muñoz, P; Ellis, P; Rigler, D; Langford, C; Blasco, MA; Carter, N; Jackson, SP
      The EMBO journal  26  2707-18  2007

      Show Abstract
      17491589 17491589

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies