05-645 | Anti-phospho-HSP27 (Ser78) Antibody, clone JBW502

05-645
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-645
      Replaces04-447
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-HSP27 (Ser78) Antibody, clone JBW502
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-phospho-HSP27 (Ser78) Antibody, clone JBW502 is an antibody against phospho-HSP27 (Ser78) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to amino acids 73-83 of human HSP27 with a C-terminus cysteine added for conjugation (YSRAL[pS]RQLSS-C)
      CloneJBW502
      HostMouse
      Specificityphosphorylated HSP27 (Ser78)
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HSPB1
      • Hs.76067
      • HSP27
      • Hsp25
      • HspB1
      • HSP28
      • CMT2F
      • HS.76067
      • DKFZp586P1322
      • HMN2B
      • SRP27
      Modifications
      • Phosphorylation
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04792 # Involved in stress resistance and actin organization.
      SIZE: 205 amino acids; 22783 Da SUBUNIT: Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin and microtubules. Interacts with HSPB8 and HSPBAP1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
      PTM: Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
      DISEASE: SwissProt: P04792 # Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant. & Defects in HSPB1 are a cause of distal hereditary motor neuropathy (dHMN) [MIM:608634]. Distal HMN is a pure motor peripheral neuropathy without sensory abnormalities.
      SIMILARITY: SwissProt: P04792 ## Belongs to the small heat shock protein (HSP20) family.
      Molecular Weight27kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on phosphorylated HSP27 in 20mg of a cell lysate prepared from MCF-7 cells that were heat shocked for 30 minutes at 42°C, cultured for an additional 16 hours at 37°C, and then stimulated with 200mM arsenite for 30 minutes
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-HSP27 (Ser78), clone JBW502 - 22174 22174
      Anti-phospho-HSP27 (Ser78), clone JBW502 - 26224 26224
      Anti-phospho-HSP27 (Ser78), clone JBW502 - 26469 26469
      Anti-phospho-HSP27 (Ser78), clone JBW502 - DAM1770282 DAM1770282
      Anti-phospho-HSP27 (Ser78), clone JBW502 -2605321 2605321
      Anti-phospho-HSP27 (Ser78), clone JBW502 -2607566 2607566
      Anti-phospho-HSP27 (Ser78), clone JBW502 -2730939 2730939

      References

      Reference overviewApplicationPub Med ID
      MK2 SUMOylation regulates actin filament remodeling and subsequent migration in endothelial cells by inhibiting MK2 kinase and HSP27 phosphorylation.
      Chang, E; Heo, KS; Woo, CH; Lee, H; Le, NT; Thomas, TN; Fujiwara, K; Abe, J
      Blood  117  2527-37  2011

      Show Abstract
      21131586 21131586
      Phosphorylation of Ser78 of Hsp27 correlated with HER-2/neu status and lymph node positivity in breast cancer.
      Zhang, D; Wong, LL; Koay, ES
      Molecular cancer  6  52  2007

      Show Abstract
      17697330 17697330
      Heat shock protein 27 association with the I kappa B kinase complex regulates tumor necrosis factor alpha-induced NF-kappa B activation.
      Park, KJ; Gaynor, RB; Kwak, YT
      The Journal of biological chemistry  278  35272-8  2003

      Show Abstract
      Kinase Assay12829720 12829720

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies