06-597-25UG | Anti-phospho-Histone H1

25 μg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, HICC, WBRbPurifiedPolyclonal Antibody
      Catalogue Number06-597-25UG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Histone H1
      Background InformationHistone H1 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H1 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H1 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      • Colcemid treated HeLa cell lysate
      PresentationImmunoaffinity Purified immunoglobulin in 0.1M Tris-glycine, PH 7.4, 0.15M NaCl, 0.05% sodium azide and 30% glycerol.
      ApplicationAnti-phospho-Histone H1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of phospho-Histone H1 & has been validated in ICC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Biological Information
      ImmunogenHyper-phosphorylated isoform of tetrahymena Histone H1 purified by cation-exchange HPLC
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityRecognizes phospho-Histone H1, Mr 32 kDa. Additional proteins were also detected, Mr 66 and 100 kDa.
      Species Reactivity
      • Bovine
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H1C
      • MGC3992
      • H1F2
      • H1.2
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16403 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
      SIZE: 213 amino acids; 21365 Da
      SIMILARITY: SwissProt: P16403 ## Belongs to the histone H1/H5 family.
      Molecular Weight32 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunoblot Analysis: 0.5-2 μg/mL detected phosphorylated Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells (Catalog # 17-306).

      Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol/5% glacial acetic acid (v/v).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information