05-817R-I | Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant

05-817R-I
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, DB, FCRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-817R-I
      DescriptionAnti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant
      Alternate Names
      • H3S10P
      • Histone H3 (phospho Ser10)
      • H3 histone, family 3A
      • H3.3A
      • H3 histone, family 3B
      • H3.3B
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, is a recombinant rabbit monoclonal antibody that detects Histone H3 phosphorylated at serine 10. This purified recombinant mAb, also known as Anti-H3S10p, is published and validated in WB, Flow Cytometry, and is specificity verified by DB.
      Key Applications
      • Western Blotting
      • Dot Blot
      • Flow Cytometry
      Application NotesDot Blot Specificity Analysis: AbSurance™ Histone Antibody Specificity Arrays (cat# 17-668) containing various unmodified and modified Histone peptides (see table) were probed with Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant 0.05ug/mL.

      Flow Cytometry Analysis: 0.25 µg/mL of this antibody detected Histone H3 phosphorylated at serine 10 in Jurkat T lymphocytes.


      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to Human Histone H3 phosphorylated at Ser10.
      EpitopePhosphorylated Ser10
      Clone63-1C-8
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityRecognizes Histone H3 phosphorylated at Serine 10.
      IsotypeIgGκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~ 17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in colcemid treated HeLa cell lysate.

      Western Blotting Analysis: 0.05 µg/mL of this antibody detected Histone H3 phosphorylated at serine 10 in 10 µg of colcemid treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant - Q2438614 Q2438614
      Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant -2689631 2689631
      Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant Monoclonal Antibody 2918970

      References

      Reference overviewPub Med ID
      Wnt/β-catenin dependent cell proliferation underlies segmented lateral line morphogenesis.
      Aman, Andy, et al.
      Dev. Biol., 349: 470-82 (2011)  349  2011

      Show Abstract
      20974120 20974120
      Regulation of chromatin structure by histone H3S10 phosphorylation.
      Johansen, Kristen M and Johansen, Jørgen
      Chromosome Res., 14: 393-404 (2006)  2006

      Show Abstract
      16821135 16821135