09-432 | Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human Antibody

09-432
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-432
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human Antibody
      Alternate Names
      • HIRS-1
      • Insulin Receptor Substrate 1
      Background InformationInsulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol.
      Applications
      ApplicationAnti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human Antibody detects level of phospho-IRS1 (Tyr608) mouse/ (Tyr612) human & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to amino acids encompassing and including phosphorylated Tyr608 in mouse and Tyr612 in human of IRS1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes phospho-IRS1 (Tyr608) mouse/ (Tyr612) human
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteTested on human. Predicted to cross react with mouse and rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IRS1
      • IRS-1
      • HIRS-1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
      SIZE: 1242 amino acids; 131591 Da
      SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.
      PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
      SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain.
      Molecular Weight~160 kDa observed. 131.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Insulin treated HEK293 cell lysate.

      Western Blotting Analysis: 1 mg/mL of this antibody detected phospho-IRS1 (Tyr608) mouse/ (Tyr612) in 10 µg of lysate from Insulin treated HEK293 cells.cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human - 2123691 2123691
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human - 2382120 2382120
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human - 2154762 2154762
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human 2890850
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human 2876222
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human - 1970363 1970363
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human - 2020904 2020904
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human - 2298360 2298360
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human - 2505949 2505949
      Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human - 2562087 2562087

      References

      Reference overviewApplicationSpeciesPub Med ID
      Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors.
      Garwood, CJ; Ratcliffe, LE; Morgan, SV; Simpson, JE; Owens, H; Vazquez-Villaseñor, I; Heath, PR; Romero, IA; Ince, PG; Wharton, SB
      Molecular brain  8  51  2015

      Show Abstract
      26297026 26297026
      Differential interaction of Apolipoprotein-E isoforms with insulin receptors modulates brain insulin signaling in mutant human amyloid precursor protein transgenic mice.
      Chan, ES; Chen, C; Cole, GM; Wong, BS
      Scientific reports  5  13842  2015

      Show Abstract
      26346625 26346625
      High-fat diet induces hepatic insulin resistance and impairment of synaptic plasticity.
      Liu, Z; Patil, IY; Jiang, T; Sancheti, H; Walsh, JP; Stiles, BL; Yin, F; Cadenas, E
      PloS one  10  e0128274  2015

      Show Abstract
      26023930 26023930
      Oxidative stress and altered lipid homeostasis in the programming of offspring fatty liver by maternal obesity.
      Alfaradhi, MZ; Fernandez-Twinn, DS; Martin-Gronert, MS; Musial, B; Fowden, A; Ozanne, SE
      American journal of physiology. Regulatory, integrative and comparative physiology  307  R26-34  2014

      Show Abstract
      Western BlottingMouse24789994 24789994
      Reduced phosphorylation of brain insulin receptor substrate and Akt proteins in apolipoprotein-E4 targeted replacement mice.
      Ong, QR; Chan, ES; Lim, ML; Cole, GM; Wong, BS
      Scientific reports  4  3754  2014

      Show Abstract
      24435134 24435134
      Artemisia scoparia extract attenuates non-alcoholic fatty liver disease in diet-induced obesity mice by enhancing hepatic insulin and AMPK signaling independently of FGF21 pathway.
      Wang, Zhong Q, et al.
      Metab. Clin. Exp., (2013)  2013

      Show Abstract
      23702383 23702383
      Muscle-specific knock-out of NUAK family SNF1-like kinase 1 (NUAK1) prevents high fat diet-induced glucose intolerance.
      Inazuka, F; Sugiyama, N; Tomita, M; Abe, T; Shioi, G; Esumi, H
      The Journal of biological chemistry  287  16379-89  2012

      Show Abstract
      Western BlottingMouse22418434 22418434
      Comparing the effects of nano-sized sugarcane fiber with cellulose and psyllium on hepatic cellular signaling in mice.
      Wang, Zhong Q, et al.
      Int J Nanomedicine, 7: 2999-3012 (2012)  2012

      Show Abstract
      22787396 22787396
      A CD36-dependent pathway enhances macrophage and adipose tissue inflammation and impairs insulin signalling.
      Kennedy, David J, et al.
      Cardiovasc. Res., 89: 604-13 (2011)  2011

      Show Abstract
      21088116 21088116
      An organic matrix-mediated processing methodology to fabricate hydroxyapatite based nanostructured biocomposites.
      Kithva PH, Grøndahl L, Kumar R, Martin D, Trau M
      Nanoscale  1  229-32. Epub 2009 Aug 13.  2009

      Show Abstract
      20644842 20644842

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies