04-894 | Anti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W, rabbit monoclonal

100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbCulture SupernatantMonoclonal Antibody
      Catalogue Number04-894
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W, rabbit monoclonal
      Product Information
      FormatCulture Supernatant
      PresentationCultured supernantant with 0.05% sodium azide
      ApplicationThis Anti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W is validated for use in WB for the detection of phospho-KDR/Flk-1/VEGFR2 (Tyr1054).
      Key Applications
      • Western Blotting
      Application NotesWestern Blot: 1:1000-1:4000 dilution for the detection of phospho-KDR/Flk-1/VEGFR2 in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide encompassing the phosphorylated tyrosine residue 1054 of human KDR/Flk-1/VEGFR2.
      SpecificityKDR/Flk-1/VEGFR2 phosphorylated on Tyrosine 1054.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NotePredicted to have broad species cross-reactivity based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDR
      • VEGFR-2
      • VEGFR2
      • VEGFR
      • CD309
      • FLK1
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35968 # Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1356 amino acids; 151527 Da
      SUBUNIT: Interacts with SHB upon VEGF activation. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P35968 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 7 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Molecular Weight152 kDa.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), -2697398 2697398
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), -2817231 2817231
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), -2820677 2820677
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), clone D1W - DAM1528585 DAM1528585
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), clone D1W - JH1774601 JH1774601
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), clone D1W - NG17017 NG17017
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), clone D1W - NG1748983 NG1748983
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), clone D1W - NG1757837 NG1757837
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), clone D1W -2508870 2508870
      Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054), clone D1W -2673033 2673033


      Reference overviewPub Med ID
      Phosphorylation of Akt and ERK1/2 is required for VEGF-A/VEGFR2-induced proliferation and migration of lymphatic endothelium.
      Dellinger, MT; Brekken, RA
      PloS one  6  e28947  2011

      Show Abstract Full Text Article
      22174934 22174934

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      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies