04-797 | Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39

04-797
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RMplex, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-797
      Replaces05-797
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39
      Alternate Names
      • Mitogen-activated protein kinase
      • Extracellular signal-regulated kinase
      • protein tyrosine kinase ERK2
      Background InformationErk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively).
      References
      Product Information
      FormatPurified
      Control
      • RIPA cell lysate from NGF stimulated PC-12 cells
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      Key Applications
      • Multiplexing
      • Western Blotting
      Application NotesWestern Blot Analysis:
      A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
      Beadlyte® Phospho-Specificity Assay:
      1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex® microspheres. (Figure B).
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated. The immunizing sequence is highly conserved among species.
      CloneAw39
      ConcentrationVariable
      HostRabbit
      SpecificityRecognizes phosphorylated MAP Kinase 1 and 2 (Erk 1&2).
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman and rat. Predicted to cross-react with many species based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • MAPK1
      • p38
      • ERK
      • P42MAPK
      • ERK2
      • p40
      • PRKM1
      • p41mapk
      • PRKM2
      • MAPK2
      • p42-MAPK
      • p41
      • ERK-2
      • ERT1
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 360 amino acids; 41390 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular WeightMAPK1 (~44 kDa) and MAPK2 (~42 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from NGF stimulated PC-12 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 - 2441925 2441925
      Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 - DAM1502443 DAM1502443
      Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 -2608736 2608736
      Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 -2650796 2650796
      Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 -2714510 2714510
      Anti-phospho-MAP Kinase 1/2 (Erk1/2) (Thr185/Tyr187), clone AW39 -2739248 2739248
      Anti-phospho-MAP Kinase 1/2 (Erk1/2) -2697396 2697396

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies