05-481 | Anti-phospho-MAP Kinase1/2 Antibody

05-481
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Av, CaWB, IP, ELISA, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-481
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-MAP Kinase1/2 Antibody
      Alternate Names
      • Extracellular signal-regulated kinase
      • Insulin-stimulated MAP2 kinase
      • MAP kinase 1
      • MAPK 1
      • Microtubule-associated protein 2 kinase
      • extracellular signal-related kinase 1
      • mitogen-activated protein kinase 3
      Background InformationThe extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The ERK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of ERK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand. Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.
      References
      Product Information
      FormatPurified
      Control
      • Included Positive Control Lysate: Catalog # 12-302, EGF-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg (20 μL) of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG1k lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose.
      Reconstitute with 1 mL H2O (15 min, RT) for a final concentration of 100 μg/mL.
      Applications
      ApplicationDetect phospho-MAP Kinase1/2 using this Anti-phospho-MAP Kinase1/2 validated for use in WB, IP, ELISA & IC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • ELISA
      • Immunocytochemistry
      Application NotesImmunoprecipitation: Use 1-10 μg per immuno-precipitation.
      Biological Information
      ImmunogenKLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. Clone: 12D4
      Cloneclone 12D4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificitySpecific for phosphorylated (activated) Erk1 and Erk2. Antibody recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2 and will not interact with the non-phosphorylated form of the proteins. Does not cross react with activated SAP Kinase 1 or 2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Avian
      • Canine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. ...
      Gene Symbol
      • MAPK3
      • MGC20180
      • ERT2
      • HUMKER1A
      • p44-MAPK
      • PRKM3
      • ERK1
      • p44erk1
      • p44mapk
      • P44MAPK
      • HS44KDAP
      • p44-ERK1
      • P44ERK1
      • ERK-1
      Modifications
      • Phosphorylation
      Purification MethodThiophilic adsoption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P27361 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 379 amino acids; 43136 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P27361 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ...
      Molecular Weight42-44 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from EGF-stimulated human A431 carcinoma cells.

      Western Blot Analysis: 0.1-1.0 μg/mL of this antibody detected phospho-MAPK in RIPA lysates from EGFstimulated human A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 2395663 2395663
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 0012S0611 0012S0611
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 0012S0612 0012S0612
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 16809 16809
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 19341 19341
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 2068209 2068209
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 20685 20685
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 21156 21156
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 21782 21782
      Anti-phospho-MAP Kinase1/2 (Erk 1/2), clone 12D4 - 2202538 2202538

      References

      Reference overviewApplicationPub Med ID
      Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease.
      D'Souza, Y; Elharram, A; Soon-Shiong, R; Andrew, RD; Bennett, BM
      Molecular brain  8  27  2015

      Show Abstract
      25910195 25910195
      MAP kinase phosphatase 1 (MKP-1/DUSP1) is neuroprotective in Huntington's disease via additive effects of JNK and p38 inhibition.
      Taylor, DM; Moser, R; Régulier, E; Breuillaud, L; Dixon, M; Beesen, AA; Elliston, L; Silva Santos, Mde F; Kim, J; Jones, L; Goldstein, DR; Ferrante, RJ; Luthi-Carter, R
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  2313-25  2013

      Show Abstract
      Western Blotting, Immunohistochemistry, Immunocytochemistry23392662 23392662
      Decreased striatal RGS2 expression is neuroprotective in Huntington's disease (HD) and exemplifies a compensatory aspect of HD-induced gene regulation.
      Seredenina, T; Gokce, O; Luthi-Carter, R
      PloS one  6  e22231  2011

      Show Abstract
      Immunofluorescence21779398 21779398
      The 5-HT 2A serotonin receptor enhances cell viability, affects cell cycle progression and activates MEK-ERK1/2 and JAK2-STAT3 signalling pathways in human choriocarcinoma cell lines.
      Oufkir T, Arseneault M, Sanderson JT, Vaillancourt C
      Placenta  31  439-47. Epub 2010 Mar 25.  2010

      Show Abstract
      20338635 20338635
      The sonic hedgehog signaling pathway is reactivated in human renal cell carcinoma and plays orchestral role in tumor growth.
      Dormoy, V; Danilin, S; Lindner, V; Thomas, L; Rothhut, S; Coquard, C; Helwig, JJ; Jacqmin, D; Lang, H; Massfelder, T
      Molecular cancer  8  123  2009

      Show Abstract
      Western Blotting20015350 20015350
      Decoy receptor 3, upregulated by Epstein-Barr virus latent membrane protein 1, enhances nasopharyngeal carcinoma cell migration and invasion.
      Ho, CH; Chen, CL; Li, WY; Chen, CJ
      Carcinogenesis  30  1443-51  2009

      Show Abstract
      19483191 19483191
      Preconditioning-mimetics bradykinin and DADLE activate PI3-kinase through divergent pathways.
      Michael V Cohen, Sebastian Philipp, Thomas Krieg, Lin Cui, Atsushi Kuno, Viktoriya Solodushko, James M Downey, Michael V Cohen, Sebastian Philipp, Thomas Krieg, Lin Cui, Atsushi Kuno, Viktoriya Solodushko, James M Downey
      Journal of molecular and cellular cardiology  42  842-51  2007

      Show Abstract Full Text Article
      17292392 17292392
      Serum- and glucocorticoid-inducible kinase (SGK) is a target of the MAPK/ERK signaling pathway that mediates memory formation in rats
      Lee, Ching-Tien, et al
      Eur J Neurosci, 23:1311-20 (2006)  2006

      16553792 16553792
      Localizing extracellular signal-regulated kinase (ERK) in pharmacological preconditioning's trigger pathway.
      Sebastian Philipp, Stuart D Critz, Lin Cui, Viktoriya Solodushko, Michael V Cohen, James M Downey
      Basic research in cardiology  101  159-67  2006

      Show Abstract
      16283591 16283591
      Hyaluronan and the interaction between CD44 and epidermal growth factor receptor in oncogenic signaling and chemotherapy resistance in head and neck cancer.
      Wang, SJ; Bourguignon, LY
      Archives of otolaryngology--head & neck surgery  132  771-8  2006

      Show Abstract
      16847188 16847188

      FAQ

      QuestionAnswer
      How can I check the phosphospecificity of my antibody once my protein is already immobilized on a membrane?You can check for the phosphospecificity of your antibody using Lambda Protein Phosphatase. Simply perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on a cell lysate and transfer the proteins to your membrane of choice. Wash the blotted nitrocellulose twice with water. Block the blotted nitrocellulose in freshly prepared TBS containing 1% bovine serum albumin (BSA) and 0.1% Triton X-100 for 1 hour at 20-25°C with constant agitation. Incubate the nitrocellulose in TBS containing 1% bovine serum albumin (BSA), 0.1% Triton X-100 and 2 mM MnCl2, and where dephosphorylation of proteins is desirable, 400 U/ml Lambda Protein Phosphatase for two hours at room temperature, or overnight at 4°C. After incubation, wash the nitrocellulose in PBS-0.1% Tween 20 for 3-5 minutes. Rinse the nitrocellulose in 4-5 changes of water. Continue with your western blotting assay.

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies