04-773 | Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal

04-773
100 µl  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M, RWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-773
      Replaces05-773
      DescriptionAnti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal
      Alternate Names
      • Myosin phosphatase target subunit 1
      • Myosin phosphatase-targeting subunit 1
      • Protein phosphatase myosin-binding subunit
      • myosin phosphatase, target subunit 1
      • protein phosphatase 1, regulatory (inhibitor) subunit 12A
      Background InformationPhosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HEK293 cell lysate phosphorylated by ROKα/ROCKII.
      PresentationCultured supernantant containing 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.
      EpitopepThr850(Ch) or pThr853(H)
      CloneSA19
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Chicken.
      Predicted to cross-react with mouse and rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMyosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine
      triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase.
      Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased
      phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin
      phosphatase through the action of Rho-kinase. Several transcript
      variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • MBS
      • MGC133042
      • MYPT1
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: Regulates myosin phosphatase activity.
      SUBUNIT STRUCTURE: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT By similarity. Binds PPP1R12B, ROCK1 and IL16.
      SUBCELLULAR LOCATION: Cytoplasm. Note: Along actomyosin filaments and stress fibers. Ref.8
      PTM: Phosphorylated by CIT (Rho-associated kinase) By similarity. Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      SEQUENCE SIMILARITIES: Contains 6 ANK repeats.
      Molecular WeightDoublet at ~130 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWesterin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-MYPT1 (Thr850), clone SA19 -
      Anti-phospho-MYPT1 (Thr850), clone SA19 - NG1915317 NG1915317
      Anti-phospho-MYPT1 (Thr850), -2578047 2578047
      Anti-phospho-MYPT1 (Thr850), -2585817 2585817
      Anti-phospho-MYPT1 (Thr850), -2593155 2593155
      Anti-phospho-MYPT1 (Thr850), -2700778 2700778
      Anti-phospho-MYPT1 (Thr850), -2779438 2779438
      Anti-phospho-MYPT1 (Thr850), clone SA19 - 2010086 2010086
      Anti-phospho-MYPT1 (Thr850), clone SA19 - 2091729 2091729
      Anti-phospho-MYPT1 (Thr850), clone SA19 - 2436428 2436428