05-1143 | Anti-phospho-Paxillin (Tyr31) Antibody, clone M102

05-1143
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1143
      ReplacesMAB1146
      DescriptionAnti-phospho-Paxillin (Tyr31) Antibody, clone M102
      Background InformationPaxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr31 and Tyr118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.
      References
      Product Information
      FormatPurified
      Control
      • Pervanadate treated L6 cell lysates
      PresentationPurified monoclonal IgG1 in phosphate-buffered saline, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.
      Applications
      ApplicationDetect phospho-Paxillin (Tyr31) using this Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenClone M102 was generated from phospho-Paxillin (Tyr31) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 31 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
      CloneM102
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody detects Paxillin phosphorylated at Tyr31.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FLJ16691
      • paxillin
      Modifications
      • Phosphorylation
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
      SUBUNIT STRUCTURE: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 By similarity. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.
      SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell junction › focal adhesion.
      PTM: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
      SEQUENCE SIMILARITIES: Belongs to the paxillin family.
      Contains 4 LIM zinc-binding domains.
      Molecular Weight72 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western Blot in pervanadate treated L6 cell lysates.
      Western Blot Analysis: 1:500 dilution of this lot detected phospho-Paxillin on 10 µg of pervanadate treated L6 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C for one year from date of receipt. Do not aliquot.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Paxillin (Tyr31), -2518470 2518470
      Anti-phospho-Paxillin (Tyr31), -2779133 2779133
      Anti-phospho-Paxillin (Tyr31), clone M102 - 2204944 2204944
      Anti-phospho-Paxillin (Tyr31), clone M102 - NG1727629 NG1727629
      Anti-phospho-Paxillin (Tyr31), clone M102 - NG1842848 NG1842848
      Anti-phospho-Paxillin (Tyr31), clone M102 - NG1915716 NG1915716
      Anti-phospho-Paxillin (Tyr31), clone M102 - NRG1701185 NRG1701185

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies