05-538 | Anti-phospho-Raf-1 (Ser338) Antibody

05-538
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-538
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Raf-1 (Ser338) Antibody
      References
      Product Information
      FormatPurified
      Control
      • Expressed in most tissue, lysates from 3T3/NIH cells stimulated with PDGF
      PresentationProtein A Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
      Applications
      ApplicationAnti-phospho-Raf-1 (Ser338) Antibody detects level of phospho-Raf-1 (Ser338) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to residues 333-345 of human Raf-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Raf-1 phosphorylated at Serine 338.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryRaf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
      Gene Symbol
      • RAF1
      • cRaf
      • Raf-1
      • c-Raf
      • RAF
      • CRAF
      • C-RAF
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
      COFACTOR: Binds 2 zinc ions per subunit.
      SIZE: 648 amino acids; 73052 Da
      SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
      Molecular Weight74 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells stimulated with PDGF
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Raf-1 (Ser338) (mouse monoclonal IgG) - 2113349 2113349
      Anti-phospho-Raf-1 (Ser338) (mouse monoclonal IgG) - 2154180 2154180
      Anti-phospho-Raf-1 (Ser338) - 20180 20180
      Anti-phospho-Raf-1 (Ser338) - 2289137 2289137
      Anti-phospho-Raf-1 (Ser338) - 23792 23792
      Anti-phospho-Raf-1 (Ser338) - 2530015 2530015
      Anti-phospho-Raf-1 (Ser338) - 27954 27954
      Anti-phospho-Raf-1 (Ser338) - 2930218 2930218
      Anti-phospho-Raf-1 (Ser338) - 32615 32615
      Anti-phospho-Raf-1 (Ser338) - DAM1661032 DAM1661032

      References

      Reference overviewApplicationSpeciesPub Med ID
      RIN1 orchestrates the activation of RAB5 GTPases and ABL tyrosine kinases to determine the fate of EGFR.
      Balaji, K; Mooser, C; Janson, CM; Bliss, JM; Hojjat, H; Colicelli, J
      Journal of cell science  125  5887-96  2012

      Show Abstract
      Western BlottingHuman22976291 22976291
      A genome-scale RNA-interference screen identifies RRAS signaling as a pathologic feature of Huntington's disease.
      Miller, JP; Yates, BE; Al-Ramahi, I; Berman, AE; Sanhueza, M; Kim, E; de Haro, M; DeGiacomo, F; Torcassi, C; Holcomb, J; Gafni, J; Mooney, SD; Botas, J; Ellerby, LM; Hughes, RE
      PLoS genetics  8  e1003042  2012

      Show Abstract
      Western Blotting23209424 23209424
      Regulation of mTORC1 signaling by pH.
      Balgi, AD; Diering, GH; Donohue, E; Lam, KK; Fonseca, BD; Zimmerman, C; Numata, M; Roberge, M
      PloS one  6  e21549  2011

      Show Abstract Full Text Article
      21738705 21738705
      BRAF(V600E) efficient transformation and induction of microsatellite instability versus KRAS(G12V) induction of senescence markers in human colon cancer cells.
      Oikonomou, E; Makrodouli, E; Evagelidou, M; Joyce, T; Probert, L; Pintzas, A
      Neoplasia (New York, N.Y.)  11  1116-31  2009

      Show Abstract
      Western Blotting19881948 19881948
      Filamin A-mediated down-regulation of the exchange factor Ras-GRF1 correlates with decreased matrix metalloproteinase-9 expression in human melanoma cells.
      Zhu, TN; He, HJ; Kole, S; D'Souza, T; Agarwal, R; Morin, PJ; Bernier, M
      The Journal of biological chemistry  282  14816-26  2007

      Show Abstract
      17389601 17389601
      Development of a highly reproducible three-dimensional organotypic model of the oral mucosa.
      Anna Dongari-Bagtzoglou, Helena Kashleva
      Nature protocols  1  2012-8  2006

      Show Abstract Full Text Article
      17487190 17487190
      Raf-1 serine 338 phosphorylation plays a key role in adhesion-dependent activation of extracellular signal-regulated kinase by epidermal growth factor.
      Edin, ML; Juliano, RL
      Molecular and cellular biology  25  4466-75  2005

      Show Abstract
      15899852 15899852
      Raf-1 kinase inhibitor protein: structure, function, regulation of cell signaling, and pivotal role in apoptosis.
      Odabaei, Golaun, et al.
      Adv. Cancer Res., 91: 169-200 (2004)  2004

      Show Abstract
      15327891 15327891
      Untying the regulation of the Raf-1 kinase.
      Dhillon, Amardeep S and Kolch, Walter
      Arch. Biochem. Biophys., 404: 3-9 (2002)  2002

      Show Abstract
      12127063 12127063
      Anti-apoptotic signaling of the insulin-like growth factor-I receptor through mitochondrial translocation of c-Raf and Nedd4
      Peruzzi, F., et al
      J Biol Chem, 276:25990-6 (2001)  2001

      Immunoblotting (Western)11352919 11352919

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies