05-368MG | Anti-phospho-Ser/Thr-Pro MPM-2 Antibody

1 mg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtELISA, IHC, IP, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-368MG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-Ser/Thr-Pro MPM-2 Antibody
      OverviewThis antibody recognizes a variety of proteins that are phosphorylated during mitosis.
      Background InformationUpon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
      Product Information
      • Colcemid-treated HeLa (lane 2) cell lysates, A431 mitotic cells fixed with 3.7% paraformaldehyde
      PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in mouse IgG in 1.0 mL PBS containing no preservatives.
      ApplicationDetect phospho-Ser/Thr-Pro MPM-2 also known as Mitotic protein #2 with Anti-phospho-Ser/Thr-Pro MPM-2 Antibody (Mouse Monoclonal Antibody), that has been demonstrated to work in ELISA, IHC, IP & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenMitotic human HeLa cell cytosolic lysate
      SpecificityRecognizes phosphorylated serines or threonines when followed by a proline.
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      • Phosphorylation
      Purification MethodProtein G Purified
      Molecular Weightvaries depending on the protein detected
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-Ser/Thr-Pro, MPM-2 (mouse monoclonal IgG1) - 2117728 2117728
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 0702051572 0702051572
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 0704056977 0704056977
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 27704 27704
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - 31404 31404
      Anti-phospho-Ser/Thr-Pro, MPM-2 (Mitotic protein monoclonal #2) - DAM1408764 DAM1408764
      Anti-phospho-Ser/Thr-Pro, MPM-2 (mouse monoclonal IgG1) 2970639
      Anti-phospho-Ser/Thr-Pro, MPM-2 (mouse monoclonal IgG1) 3115881
      Anti-phospho-Ser/Thr-Pro, MPM-2 (mouse monoclonal IgG1) - 2328345 2328345
      Anti-phospho-Ser/Thr-Pro, MPM-2 - 2424745 2424745


      Reference overviewApplicationPub Med ID
      Prdx4 is a compartment-specific H2O2 sensor that regulates neurogenesis by controlling surface expression of GDE2.
      Yan, Y; Wladyka, C; Fujii, J; Sockanathan, S
      Nature communications  6  7006  2015

      Show Abstract
      25943695 25943695
      Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation.
      Whalley, HJ; Porter, AP; Diamantopoulou, Z; White, GR; Castañeda-Saucedo, E; Malliri, A
      Nature communications  6  7437  2015

      Show Abstract
      26078008 26078008
      Sequence-specific and phosphorylation-dependent proline isomerization: a potential mitotic regulatory mechanism.
      Yaffe, M B, et al.
      Science, 278: 1957-60 (1997)  1997

      Show Abstract
      Immunoblotting (Western)9395400 9395400
      Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope.
      Westendorf, J M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 91: 714-8 (1994)  1994

      Show Abstract
      8290587 8290587
      The mitosis-specific monoclonal antibody MPM-2 recognizes phosphoproteins associated with the nuclear envelope in Chlamydomonas reinhardtii cells.
      Harper, J D, et al.
      Eur. J. Cell Biol., 51: 272-8 (1990)  1990

      Show Abstract
      1693575 1693575
      Mitosis-specific monoclonal antibody MPM-2 inhibits Xenopus oocyte maturation and depletes maturation-promoting activity.
      Kuang, J, et al.
      Proc. Natl. Acad. Sci. U.S.A., 86: 4982-6 (1989)  1989

      Show Abstract
      2662192 2662192
      Phosphoproteins are components of mitotic microtubule organizing centers.
      Vandre, D D, et al.
      Proc. Natl. Acad. Sci. U.S.A., 81: 4439-43 (1984)  1984

      Show Abstract
      6379644 6379644
      Monoclonal antibodies to mitotic cells.
      Davis, F M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 80: 2926-30 (1983)  1983

      Show Abstract
      6574461 6574461

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      05-368 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody Show Pricing & Availability
      05-368-KC Anti-phospho-Ser/Thr-Pro, MPM-2 (KC) Show Pricing & Availability

      Alternative Format

      Catalogue Number Description  
      16-155 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, FITC conjugate Show Pricing & Availability
      16-220 Anti-phospho-Ser/Thr-Pro MPM-2 Antibody, Cy5 conjugate Show Pricing & Availability

      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies