07-1248 | Anti-phospho-Tpx2 (Ser738) Antibody

07-1248
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Rhesus MacaqueWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1248
      DescriptionAnti-phospho-Tpx2 (Ser738) Antibody
      Alternate Names
      • TPX2, microtubule-associated, homolog (Xenopus laevis)1 2 chromosome 20 open reading frame 1
      • Protein fls353
      • preferentially expressed in colorectal cancer
      • Restricted expression proliferation-associated protein 100
      • Differentially expressed in cancerous and non-cancerous lung cells 2
      • TPX2, microtubule-associated protein homolog
      • Hepatocellular carcinoma-associated antigen 519
      • restricted expression proliferation associated protein 100
      • differentially expressed in lung cells
      • targeting protein for XKLP2
      Background InformationTargeting protein for Xklp2 (Tpx2) is a major spindle assembly factor belonging to the TPX2 family of proteins. Tpx2 is a required component in the normal assembly of mitotic spindles and, during apoptosis, for microtubules. It is also necessary to the nucleation of microtubules in a chromatin and/or kinetechore dependent manner. Tpx2 activates Aurora-A kinase through promotion of autophosphorylation, protects the activated kinase from dephosphorylation, and regulates its localization to spindle microtubules. Normal expression of Tpx2 is exclusive to proliferating cells, specifically those transitioning from G1/S to the termination of cytokinesis. Tissue expression has only been observed in lung cancer cell lines. Phosphorylation of Tpx2 occurs on serine residues; however, the purpose of the modification has yet to be determined.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Lambda phosphatase untreated and treated HeLa cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect phospho-Tpx2 (Ser738) using this Anti-phospho-Tpx2 (Ser738) Antibody validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A previous lot was used by an independent laboratory in BT549 breast cancer cells. (Dr. Shoukat Dedhar, BC Cancer Research Centre, Vancouver, BC, Canada, V5Z 1L3, sdedhar@interchange.ubc.ca)
      Biological Information
      ImmunogenLinear peptide corresponding to human Tpx2 phosphorylated at Ser738.
      EpitopePhosphorylated Ser738
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Tpx2 phosphorylated at Ser738.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with rhesus macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TPX2
      • DIL-2
      • HCTP4
      • p100
      • FLS353
      • HCA519
      • DIL2
      • REPP86
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.

      SUBUNIT STRUCTURE: Interacts with AURKA.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Note: During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules. TISSUE SPECIFICTY: Expressed in lung carcinoma cell lines but not in normal lung tissues.

      DEVELOPMENTAL STAGE: Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

      SEQUENCE SIMILARITIES: Belongs to the TPX2 family.
      Molecular Weight~ 95 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in lambda phosphatase treated and untreated HeLa cell lysates.

      Western Blot Analysis: 1 µg/mL of this antibody detected Tpx2 on 10 µg of lambda phosphatase treated and untreated HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies