04-1009 | Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal

04-1009
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1009
      DescriptionAnti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal
      Alternate Names
      • acetyl-CoA carboxylase 1
      • acetyl-CoA carboxylase-alpha
      • acetyl-Coenzyme A carboxylase alpha
      Background InformationAcetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.
      References
      Product Information
      FormatUnpurified
      Control
      • Untreated and LP-treated A431 cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.
      Biological Information
      ImmunogenPhospho-specific peptide corresponding to residues surrounding Ser79 of human ACC1.
      EpitopePhosphorylated Ser79
      CloneEP1885Y
      HostRabbit
      SpecificityThis antibody detects ACC1 phosphorylated at Ser79.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAcetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriche in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • ACAC
      • ACC
      • ACC-alpha
      • ACC1
      • ACCA
      • EC 6.4.1.2
      • OTTHUMP00000164069
      Modifications
      • Phosphorylation
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

      CATALYTIC ACTIVITY: ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.

      ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-carboxyl-carrier protein.

      COFACTOR: Biotin.

      Binds 2 manganese ions per subunit.

      ENZYME REGULATION: By phosphorylation By similarity.

      PATHWAY: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.

      SUBUNIT STRUCTURE: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.

      SUBCELLULAR LOCATION: Cytoplasm.

      TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

      PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.

      INVOLVEMENT IN DISEASE: Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth.

      SEQUENCE SIMILARITIES: Contains 1 ATP-grasp domain.

      Contains 1 biotin carboxylation domain.

      Contains 1 biotinyl-binding domain.

      Contains 1 carboxyltransferase domain.

      Molecular Weight~280 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and LP-treated A431 cell lysates.
      Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect ACC1 in untreated and LP-treated A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal - 2420361 2420361
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal - 2227193 2227193
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal - 2266787 2266787
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal - JBC1787340 JBC1787340
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal - JBC1787341 JBC1787341
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal - NG1741711 NG1741711
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal - NG1810506 NG1810506
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal - NG1865056 NG1865056
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal -2529875 2529875
      Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79), clone EP1885Y, Rabbit Monoclonal -2537791 2537791

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies