07-823 | Anti-phospho eIF4E (Ser209) Antibody
, and probed with anti-phospho-eIF4E (Ser209) for 2 hours at room temperature. Lane 2 was pre-blocked with blocking peptide corresponding to phospho-eIF4E (Ser209). Lane 3 was pre-incubated with lambda phosphatase prior to primary antibody incubation. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phosphorylated eIF4E.','<b>Immunoblot Analysis</b> Lysate from HeLa cells was resolved by electrophoresis, transferred to PV','1','/waroot/thumbnail/07-823wb[07-823wb-ALL].jpg'))
Anti-phospho eIF4E (Ser209) Antibody is an antibody against phospho eIF4E (Ser209) for use in WB.
More>> Anti-phospho eIF4E (Ser209) Antibody is an antibody against phospho eIF4E (Ser209) for use in WB. Less<<Anti-phospho eIF4E (Ser209) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Overview
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Key Spec Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
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| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
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| Catalogue Number | 07-823 |
| Brand Family | Upstate |
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| Description | Anti-phospho eIF4E (Ser209) Antibody |
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| Format | Affinity Purified |
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| Application | Anti-phospho eIF4E (Ser209) Antibody is an antibody against phospho eIF4E (Ser209) for use in WB. |
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| Material Size | 100 µL |
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Documentation
SDS
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Certificates of Analysis
References
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| Influence of electric charge variation at residues 209 and 159 on the interaction of eIF4E with the mRNA 5' terminus Zuberek, Joanna, et al Biochemistry, 43:5370-9 (2004) 2004 | 15122903
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| Phosphorylation of eukaryotic initiation factor 4E markedly reduces its affinity for capped mRNA Scheper, Gert C, et al J Biol Chem, 277:3303-9 (2002) 2002 | 11723111
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| Modifications of eukaryotic initiation factor 4F (eIF4F) in adult cardiocytes by adenoviral gene transfer: differential effects on eIF4F activity and total protein synthesis rates Saghir, A N, et al Biochem J, 356:557-66 (2001) 2001 | 11368785
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| Phosphorylation of eukaryotic initiation factor 4E (eIF4E) at Ser209 is not required for protein synthesis in vitro and in vivo McKendrick, L, et al Eur J Biochem, 268:5375-85 (2001) 2001 | 11606200
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| Phosphorylation of the cap-binding protein eukaryotic translation initiation factor 4E by protein kinase Mnk1 in vivo Waskiewicz, A. J. et al. Mol. Cell Biol., 19(3):1871-1880 (1999) 1999 | 10022874
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| Phosphorylation of eIF-4E on serine 209 by protein kinase C is inhibited by the translational repressors, 4E-binding proteins Whalen, S. G. et al. J. Biol. Chem., 271(20):11831-11837 (1996) 1996 | 8662663
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| Serine 209, not serine 53, is the major site of phosphorylation in initiation factor eIF-4E in serum-treated Chinese hamster ovary cells Flynn, A and Proud, C G J Biol Chem, 270:21684-8 (1995) 1995 | 7665584
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| Phosphorylation of eukaryotic protein synthesis initiation factor 4E at Ser-209 Joshi, B, et al J Biol Chem, 270:14597-603 (1995) 1995 | 7782323
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Brochure
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| RNA-Binding Protein Immunoprecipitation |