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07-823 Anti-phospho eIF4E (Ser209) Antibody

Anti-phospho eIF4E (Ser209) Antibody is an antibody against phospho eIF4E (Ser209) for use in WB.

Anti-phospho eIF4E (Ser209) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling, RNA Binding Protein (RBP) Gene Symbol: EIF4E, eIF4E, CBP, eIF-4E, MGC111573, EIF-4E, EIF4E1, EIF4F, EIF4EL1 UniProt Number: P06730
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07-823
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-823
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho eIF4E (Ser209) Antibody
Alternate Names
  • eukaryotic translation initiation factor 4E
  • CBP
  • EIF4EL1
  • EIF4F
References
Product Information
FormatAffinity Purified
Quality LevelMQ100
Applications
ApplicationAnti-phospho eIF4E (Ser209) Antibody is an antibody against phospho eIF4E (Ser209) for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH conjugated synthetic peptide corresponding to amino acids surrounding Ser209 of human eIF4E. Sequence conserved in mouse and rat.
HostRabbit
SpecificityRecognizes eIF4E phosphorylated on Ser209.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteMouse, rat and rabbit (100% homologous) eIF4E have not been tested but are expected to react.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAll eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]
Gene Symbol
  • EIF4E
  • eIF4E
  • CBP
  • eIF-4E
  • MGC111573
  • EIF-4E
  • EIF4E1
  • EIF4F
  • EIF4EL1
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P06730 # Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
SIZE: 217 amino acids; 25097 Da
SUBUNIT: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP- kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 and EIF4A2. Interacts with NGDN (By similarity). Interacts with Lassa virus Z protein.
PTM: Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
SIMILARITY: SwissProt: P06730 ## Belongs to the eukaryotic initiation factor 4E family.
Molecular Weight25 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-823 04053252403798

Documentation

Anti-phospho eIF4E (Ser209) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho eIF4E (Ser209) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-eIF4E (Ser209) (Affinity Purified Rabbit Polyclonal IgG) - DAM1457528 DAM1457528
Anti-phospho-eIF4E (Ser209) (Affinity Purified Rabbit Polyclonal IgG) - DAM1482749 DAM1482749
Anti-phospho-eIF4E (Ser209) - DAM1514178 DAM1514178
Anti-phospho-eIF4E (Ser209) - DAM1524063 DAM1524063
Anti-phospho-eIF4E (Ser209) - DAM1541121 DAM1541121
Anti-phospho-eIF4E (Ser209) - DAM1601813 DAM1601813
Anti-phospho-eIF4E (Ser209) - JBC1863389 JBC1863389
Anti-phospho-eIF4E (Ser209) - JBC1872110 JBC1872110
RABBIT ANTI-eIF4E (pS209) PHOSPHOSPECIFIC POLYCLONAL ANTIBODY - 2117986 2117986
RABBIT ANTI-eIF4E (pS209) PHOSPHOSPECIFIC POLYCLONAL ANTIBODY - 2120007 2120007
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References

Reference overviewPub Med ID
Influence of electric charge variation at residues 209 and 159 on the interaction of eIF4E with the mRNA 5' terminus
Zuberek, Joanna, et al
Biochemistry, 43:5370-9 (2004)  2004

15122903 15122903
Phosphorylation of eukaryotic initiation factor 4E markedly reduces its affinity for capped mRNA
Scheper, Gert C, et al
J Biol Chem, 277:3303-9 (2002)  2002

11723111 11723111
Phosphorylation of eukaryotic initiation factor 4E (eIF4E) at Ser209 is not required for protein synthesis in vitro and in vivo
McKendrick, L, et al
Eur J Biochem, 268:5375-85 (2001)  2001

11606200 11606200
Modifications of eukaryotic initiation factor 4F (eIF4F) in adult cardiocytes by adenoviral gene transfer: differential effects on eIF4F activity and total protein synthesis rates
Saghir, A N, et al
Biochem J, 356:557-66 (2001)  2001

11368785 11368785
Phosphorylation of the cap-binding protein eukaryotic translation initiation factor 4E by protein kinase Mnk1 in vivo
Waskiewicz, A. J. et al.
Mol. Cell Biol., 19(3):1871-1880 (1999)  1999

10022874 10022874
Phosphorylation of eIF-4E on serine 209 by protein kinase C is inhibited by the translational repressors, 4E-binding proteins
Whalen, S. G. et al.
J. Biol. Chem., 271(20):11831-11837 (1996)  1996

8662663 8662663
Phosphorylation of eukaryotic protein synthesis initiation factor 4E at Ser-209
Joshi, B, et al
J Biol Chem, 270:14597-603 (1995)  1995

7782323 7782323
Serine 209, not serine 53, is the major site of phosphorylation in initiation factor eIF-4E in serum-treated Chinese hamster ovary cells
Flynn, A and Proud, C G
J Biol Chem, 270:21684-8 (1995)  1995

7665584 7665584

Brochure

Title
RNA-Binding Protein Immunoprecipitation