07-823 | Anti-phospho eIF4E (Ser209) Antibody

07-823
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-823
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho eIF4E (Ser209) Antibody
      Alternate Names
      • eukaryotic translation initiation factor 4E
      • CBP
      • EIF4EL1
      • EIF4F
      References
      Product Information
      FormatAffinity Purified
      Applications
      ApplicationAnti-phospho eIF4E (Ser209) Antibody is an antibody against phospho eIF4E (Ser209) for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH conjugated synthetic peptide corresponding to amino acids surrounding Ser209 of human eIF4E. Sequence conserved in mouse and rat.
      HostRabbit
      SpecificityRecognizes eIF4E phosphorylated on Ser209.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteMouse, rat and rabbit (100% homologous) eIF4E have not been tested but are expected to react.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAll eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]
      Gene Symbol
      • EIF4E
      • eIF4E
      • CBP
      • eIF-4E
      • MGC111573
      • EIF-4E
      • EIF4E1
      • EIF4F
      • EIF4EL1
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06730 # Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
      SIZE: 217 amino acids; 25097 Da
      SUBUNIT: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP- kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 and EIF4A2. Interacts with NGDN (By similarity). Interacts with Lassa virus Z protein.
      PTM: Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
      SIMILARITY: SwissProt: P06730 ## Belongs to the eukaryotic initiation factor 4E family.
      Molecular Weight25 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-eIF4E (Ser209) (Affinity Purified Rabbit Polyclonal IgG) - DAM1457528 DAM1457528
      Anti-phospho-eIF4E (Ser209) (Affinity Purified Rabbit Polyclonal IgG) - DAM1482749 DAM1482749
      Anti-phospho-eIF4E (Ser209) - DAM1514178 DAM1514178
      Anti-phospho-eIF4E (Ser209) - DAM1524063 DAM1524063
      Anti-phospho-eIF4E (Ser209) - DAM1541121 DAM1541121
      Anti-phospho-eIF4E (Ser209) - DAM1601813 DAM1601813
      Anti-phospho-eIF4E (Ser209) - JBC1863389 JBC1863389
      Anti-phospho-eIF4E (Ser209) - JBC1872110 JBC1872110
      RABBIT ANTI-eIF4E (pS209) PHOSPHOSPECIFIC POLYCLONAL ANTIBODY - 2117986 2117986
      RABBIT ANTI-eIF4E (pS209) PHOSPHOSPECIFIC POLYCLONAL ANTIBODY - 2120007 2120007

      References

      Reference overviewPub Med ID
      Influence of electric charge variation at residues 209 and 159 on the interaction of eIF4E with the mRNA 5' terminus
      Zuberek, Joanna, et al
      Biochemistry, 43:5370-9 (2004)  2004

      15122903 15122903
      Phosphorylation of eukaryotic initiation factor 4E markedly reduces its affinity for capped mRNA
      Scheper, Gert C, et al
      J Biol Chem, 277:3303-9 (2002)  2002

      11723111 11723111
      Modifications of eukaryotic initiation factor 4F (eIF4F) in adult cardiocytes by adenoviral gene transfer: differential effects on eIF4F activity and total protein synthesis rates
      Saghir, A N, et al
      Biochem J, 356:557-66 (2001)  2001

      11368785 11368785
      Phosphorylation of eukaryotic initiation factor 4E (eIF4E) at Ser209 is not required for protein synthesis in vitro and in vivo
      McKendrick, L, et al
      Eur J Biochem, 268:5375-85 (2001)  2001

      11606200 11606200
      Phosphorylation of the cap-binding protein eukaryotic translation initiation factor 4E by protein kinase Mnk1 in vivo
      Waskiewicz, A. J. et al.
      Mol. Cell Biol., 19(3):1871-1880 (1999)  1999

      10022874 10022874
      Phosphorylation of eIF-4E on serine 209 by protein kinase C is inhibited by the translational repressors, 4E-binding proteins
      Whalen, S. G. et al.
      J. Biol. Chem., 271(20):11831-11837 (1996)  1996

      8662663 8662663
      Serine 209, not serine 53, is the major site of phosphorylation in initiation factor eIF-4E in serum-treated Chinese hamster ovary cells
      Flynn, A and Proud, C G
      J Biol Chem, 270:21684-8 (1995)  1995

      7665584 7665584
      Phosphorylation of eukaryotic protein synthesis initiation factor 4E at Ser-209
      Joshi, B, et al
      J Biol Chem, 270:14597-603 (1995)  1995

      7782323 7782323

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies