09-213 | Anti-phospho-mTOR (Ser2448) Antibody

09-213
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-213
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-mTOR (Ser2448) Antibody
      Alternate Names
      • FRAP1
      • FRAP
      • FRAP2
      • RAFT1
      • RAPT1
      • FK506 binding protein 12-rapamycin associated protein 1
      Background InformationmTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GβL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • EGF-treated and untreated A431 cell lysate.
      PresentationPurified rabbit polyclonal IgG in storage buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-mTOR Antibody is validated for use in WB for the detection of phospho-mTOR (Ser2448).
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenAmino acids encompassing and including phosphorylated Ser2448 of human mTOR
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes mTOR when phosphorylated on Ser2448
      Species Reactivity
      • Human
      Species Reactivity NoteTested to react with Human.
      Predicted to cross react with rat, mouse, sheep & Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.
      Gene Symbol
      • FRAP1
      • RAPT1
      • mTOR
      • FRAP
      • MTOR
      • FLJ44809
      • FRAP2
      • RAFT1
      • FRAP1 FK506 binding protein 12-rapamycin associated protein 1
      • mammalian target of rapamycin
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
      SIZE: 2549 amino acids; 288892 Da
      SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.
      TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
      SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.
      Molecular Weight~289 kDa observed.
      An uncharacterized band may be obsereved at ~59 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on EGF-treated and untreated A431 cell lysate.

      Western Blot Analysis:
      This antibody detected mTOR phosphorylated on Ser2448 in EGF-treated and untreated A431 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of shipment.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-mTOR (Ser2448) (rabbit polyclonal) Polyclonal Antibody Q2922237
      Anti-phospho-mTOR (Ser2448) - 1984828 1984828
      Anti-phospho-mTOR (Ser2448) - 2299579 2299579
      Anti-phospho-mTOR (Ser2448) - DAM1503827 DAM1503827
      Anti-phospho-mTOR (Ser2448) - DAM1636935 DAM1636935
      Anti-phospho-mTOR (Ser2448) - DAM1754405 DAM1754405
      Anti-phospho-mTOR (Ser2448) - DAM1774733 DAM1774733
      Anti-phospho-mTOR (Ser2448) - DAM1787930 DAM1787930
      Anti-phospho-mTOR (Ser2448) - DAM1805276 DAM1805276
      Anti-phospho-mTOR (Ser2448) - JBC1900532 JBC1900532

      References

      Reference overviewApplicationPub Med ID
      SWI/SNF chromatin remodeling enzyme ATPases promote cell proliferation in normal mammary epithelial cells.
      Cohet, N; Stewart, KM; Mudhasani, R; Asirvatham, AJ; Mallappa, C; Imbalzano, KM; Weaver, VM; Imbalzano, AN; Nickerson, JA
      Journal of cellular physiology  223  667-78  2010

      Show Abstract
      Western Blotting20333683 20333683

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies